Gel Filtration – Which column to choose for your size exclusion purification?

Hand with Antibody V2-01Gel filtration (GF), also referred as Size Exclusion Chromatography (SEC), plays a key role in the high quality purification of enzymes, polysaccharides, nucleic acids, proteins and other biological macromolecules. Gel filtration is the simplest and mildest of all chromatography techniques to separate biomolecules on the basis of difference in size.  Nevertheless, the list of available columns is quite awesome and the characteristics of each of them are very different. Also, each characteristic highly influences the quality of the final purified product with important consequences in downstream applications.

I’ve put together a simple guide to help you find your GF system, together with some tips and tricks based on my experience when producing recombinant proteins and antibodies for our clients involved in the early R&D stages through to the latest phases of the bio-production flow.

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Written by Isabelle Topin, PhD
Isabelle Topin was a Project Manager at tebu-bio - her expertise ranges from genes to proteins, through biochemistry and molecular, cellular, structural and animal biology.