DyLight™ will be your routine fluorescent dye
Rockland Immunochemicals have developed a suite of fluorescent dyes (DyLight™) with unique fluorescence intensity and photostability. Here, we take a look at how DyLight™ dyes have all the characteristics and potential to become your daily fluorescent dye conjugates.
Mouse IgG (H&L) Antibody DyLight™680 high fluorescence intensity and photostability vs. IRDye®700.
#1- What makes DyLight™ dyes unique?
DyLight™ conjugates are ideal substitutes for IRDye® products as far as experimental results and budgets are concerned. For example, DyLight™ 680 and DyLight™ 800 products are an excellent alternative to Infra-Red and Near Infra-Red IRDye® products.
DyLight dyes and fluorescent emission spectra. Source: Rockland Immuniochemicals, tebu-bio.
#2- Are DyLight™ dyes compatible with common fluorescence instrumentation?
#3- What range of applications do DyLight™ dyes cover?
DyLight™ conjugated antibodies are ideal for TrueBlot® IP / Western blotting, Multiplex imaging, Flow cytometry, IF, ELISA, High Content screening, High resolution microscopy…
DyLight™ Dyes used in double-WB labeling in fluorescent WB (e.g. using a Li-Cor Odyssey).
We’ve noted that these are some of the most popular Dylight™ products:
- Anti-MOUSE IgG (H&L) (DONKEY) Antibody DyLight™ 800 Conjugated Pre-Adsorbed (cat. nr 039610-745-124)
- Anti-MOUSE IgG (H&L) (GOAT) Antibody DyLight™ 800 Conjugated Pre-Adsorbed (cat. nr 039610-145-121)
- Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 800 Conjugated Pre-Adsorbed (cat. nr 039611-145-122)
- Anti-MOUSE IgG (H&L) (GOAT) Antibody DyLight™ 680 Conjugated Pre-Adsorbed (cat. nr 039610-144-121)
Anti-histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
Well… has this given you any ideas, would you like to learn more or try Rockland’s DyLight™ fluorescent conjugated products?
Get in touch with tebu-bio’s experts, or leave a message below.
Fluorescent dyes used for primary and secondary antibody conjugation with respective colours and spectra. Source: Rockland Immunochemicals, tebu-bio.
Do you have a Dylight conjugation kit? I want to prepare Delight antiserum(without purifying IgG).
Dear Masamichi,
Thank you for your question.
As far as the DyLight technology is concerned, the trend is to provide high quality conjugated antibodies validated for their applications. Each lot produced is thus tested and QCed according to internal standards and is released only if it meets these standards.
For protein labeling, various ready-to-use conjugation kit are available. They allow labeling of your own samples with fluorescent dyes like HiLyte, FITC… but not yet with DyLight. Our expert will contact you directly by e-mail to provide you with all the information needed to assist you in the conjugation of your preparations.
Best regards,
Philippe