CRISPR/Cas9 vector delivery maximized with DNA-In CRISPR

CRISPR/Cas 9 Genome editing and Engineering

CRISPR-Cas9 is a popular method that brings researchers endless experimental strategies to create their own research-based cellular models. In this post we’ll review a new transfection reagent especially engineered to maximize Cas9 vectors deliveries inside cells with low cellular toxicity.

Cas9-GFP expression vector delivery in human primary fibroblasts with DNA-In®

Cas9-GFP expression vector delivery in human primary fibroblasts with DNA-In®.

Whatever the experimental objectives (gene knock-out or knock-in, single point mutation(s), promoter swapping, protein truncation…) and applications (Drug discovery, Cell signaling, Phenotypic assays…), CRISPR gene editing methods require extremely robust reagents (cell lines and sgRNA, Cas9 mRNA, anti-Cas9 or anti-GFP antibodies, …).

MTI-GlobalStem has released a new high quality transfection reagent to deliver Cas9 expression vectors or Cas9/sgRNA expression vectors: the DNA-In® CRISPR transfection reagent.

DNA-In® CRISPR has been developed for the delivery of large CRISPR/Cas9 plasmid expression vectors into a broad range of cell types. Successfully used on primary cells and cell lines (Human primary fibroblasts, keratinocytes, endothelial cells (HuVEC), skeletal muscle cells, HeLa cells and C2C12 mouse myoblasts… to name but a few!), the animal-free DNA-In® CRISPR achieves the highest number of cells transfected in a population without toxicity.

Cas9-GFP expression vector delivery in HeLa cells with DNA-In®

Cas9-GFP expression vector delivery in HeLa cells with DNA-In®.

This reagent is ideal for intracellular delivery of DNA into cells in the presence of serum at a cell density of 50% to 70%.

Download the optimized DNA-In® CRISPR Transfection Reagent protocol here.

Want to know more about DNA-In® CRISPR Transfection Reagent? Looking for tailored made solutions in relation to your CRISPR/CAS9-based experiments? Leave us a message below.

tebu-bio’s exoerts advise you in the design of your genome editing experiments and provide you with user-friendly solutions suited to your cellular models.

ICC/IF analysis of HeLa cells transiently transfected with flag-tagged Cas9

IF of HeLa cells transfected with flag-tagged Cas9 using anti-Cas9 (GeneTex cat. nr 210GTX53807 – green) and DAPI (blue).

IF with anti-GFP (cat nr 600-101-215) on Sf-1Cre mice crossed to the ZEG reporter line (brain).

IF with anti-GFP (cat nr 039600-101-215) on Sf-1 Cre mice crossed to the ZEG reporter line (brain).

Written by Philippe Fixe, PhD
Philippe Fixe is a former Marketing Manager at tebu-bio, passionate about innovation and R&D in Life sciences, Biotechnology, Medical research, Drug discovery, and also a keen photographer.