The ubiquitin-proteasome system (UPS) is a well-characterized protein degradation system in cells whose dysfunction is implicated in many diseases, including neurodegeneration and cancer^1,2. Major UPS components are ubiquitin (Ub), Ub ligases, Ub hydrolases (deubiquitinases [DUBs]), and the proteasome.

Many research labs would like to acquire and implement the CRISPR-Cas9 technology for their gene editing projects. Indeed, it’s a powerful system based on a simple principle: an endonuclease, the CAS9, is driven onto a target site by a short guide RNA. There are so many strategies with benefits and drawbacks that is quite challenging to figure how to start out. Newcomers may be pushed into necessarily becoming experts before finding an efficient way to success. But what if you could use a complete and simple kit to facilitate your projects?

During the past decade, mass spectrometry, notably LC/MS, has become a major approach to identify and quantify proteins in patient samples. It includes analysis before treatments to find the proper biomarkers for cancers diagnostics, and monitoring of the arising of the anti-drug antibodies in human plasma after therapies. Despite the fact that MS has pretty high sensitivity and specificity, research in biotherapeutics requires even more. Immunocapture, and so specific protein enrichment, offer an interesting immunoassay solution. Here, we present simple to use and efficient reagents to improve the immunocapture and thus the output of the MS technology.

Pooled human hepatocytes are a preferred test system in many drug discovery and development applications requiring intact cellular systems for in vitro testing. Intact hepatocytes contain the major hepatic drug-metabolizing enzymes and co-factors required to effectively evaluate the metabolism and potential drug-drug interactions of drug candidates.

Over the last decade, improvements in cryopreservation technologies have made using cryopreserved human hepatocytes more convenient. Pooled cryopreserved hepatocytes reduce the inter-individual differences and polymorphic distribution of liver enzymes. However, it’s crucial to carefully select a pool according to its performance but also the application it is to be used for.