Tissue culture for Primary cell isolation are often jeopardized by the outgrowth of contaminating fibroblasts. As fibroblasts usually grow at a much faster rate compared to the target cell population, their expansion has to be tightly controlled to end up with a culture as pure as possible.
The iQuant kits developped by ABP Bioprobes provide accurate quantitation of DNA and RNA with a wide range including high sensitivity. They are useful for NGS applications, qPCR analysis, and in vitro transcription to produce RNA.
Fluorescence-based DNA and RNA quantitation using the iQuant kits is highly sensitive. It is also specific for DNA or RNA providing more accurate concentration in the presence of common contaminants, including free nucleotides, protein, detergents and salts.
Tetramethylbenzidine (TMB) is a classic substrate for peroxidase activity that is used for microwell plate analysis such as an ELISA test. A quantitative color change from yellow to blue reveals the activity and can be read at 620-650 nm for ELISA test.
Surmodics have developed several types of TMB, for different purposes.