Glutathione (GSH) is a tri-peptide (Glutamic acid, Cystein and Glycine) present in cells, showing an antioxidant effect allowing the reduction and the removal of Reactive Oxygen Species (ROS) and Peroxide from the cell. The new QuicGSHS3.0 probe from Goryo Chemicals will allow you to easily quantify intracellular concentration of GSH by Fluorescent Live cell Imaging.
The ubiquitin-proteasome system (UPS) is a well-characterized protein degradation system in cells whose dysfunction is implicated in many diseases, including neurodegeneration and cancer1,2. Major UPS components are ubiquitin (Ub), Ub ligases, Ub hydrolases (deubiquitinases [DUBs]), and the proteasome.
RNAi offer >70% knock-down of the gene expression and thanks to CRISPR-CAS9 gene editing it is possible make a complete knock-out. The TUNR system comes to offer the possibility to have intermediates with high, medium and low reduction of the gene expression and with the same quality of targeting than the knock-out.
Many research labs would like to acquire and implement the CRISPR-Cas9 technology for their gene editing projects. Indeed, it’s a powerful system based on a simple principle: an endonuclease, the CAS9, is driven onto a target site by a short guide RNA. There are so many strategies with benefits and drawbacks that is quite challenging to figure how to start out. Newcomers may be pushed into necessarily becoming experts before finding an efficient way to success. But what if you could use a complete and simple kit to facilitate your projects?
Early diagnostic of cancers is crucial for patients and the success of their therapies. Finding a diagnostic index with high sensitivity at the early stage of cancers is a major concern. Invasive biopsies may be already too late and are certainly painful. Thus, the challenge is to find biomarkers from simple blood samples. Circulating microRNA are the best leads for such research. Let’s see why with a recent publication.
During the past decade, mass spectrometry, notably LC/MS, has become a major approach to identify and quantify proteins in patient samples. It includes analysis before treatments to find the proper biomarkers for cancers diagnostics, and monitoring of the arising of the anti-drug antibodies in human plasma after therapies. Despite the fact that MS has pretty high sensitivity and specificity, research in biotherapeutics requires even more. Immunocapture, and so specific protein enrichment, offer an interesting immunoassay solution. Here, we present simple to use and efficient reagents to improve the immunocapture and thus the output of the MS technology. [Read more…]
Protein purification from cells or supernatants, endogenous proteins complex analysis, and targeted protein enrichment for even more sensitive mass spectrometry-based biomarker discovery requires a particular immunoassay called immunoprecipitation (IP). Basically, the most robust method to pull-down a specific protein with its partners from cell lysates should be the combination of an antibody conjugated to biotin and magnetic beads with streptavidin. Unfortunately, if your antibody is not yet conjugated to biotin, conjugation is a blind process. That is why, using protein A/G coated sepharose beads is the most popular method.
But what if an antibody biotin conjugation kit could provide simple UV-scan based quantification of the conjugation yield?
In one of my previous posts, I wrote about high density glass slide antibody arrays services.
Here, I’d like to focus on another antibody microarray product line, the C-series from Raybiotech, based on a membrane antibody array technology. Similar to western blotting – therefore very easy to process – they are suitable to most research laboratories for targeted protein profiling.
Over the last years, mRNA-based experimental approaches have been successfully used in a broad range of research applications (genome editing, gene replacement, vaccine, immunotherapy, cellular reprogramming…) without risk of integration into the genome of the host cell.
mRNA molecules, mimicking fully processed, capped and polyadenylated mRNA, can now be reliably produced via in vitro transcription methods while reducing innate immune stimulation. In this post, 4 tips & Tricks are presented to help produce and optimally use your mRNAs in your research and drug development projects.