Are you looking for a simple means to boost the efficiency of gene editing and also to reduce the off-target? Yes, it does exist – in one word: 4-hydroxy Tamoxifen. That’s the HIT-CAS9.

Hybrid drug inducible CRISPR/Cas9 technology, HIT-Cas9

Zhao et al revealed tight and effective drug control of the CRISPR Cas9 based on 4-Hydroxytamoxifen. doi: 10.1016/j.omtn.2018.08.022

They built a fusion between a mutated human estrogen receptor (ERT2) and the Cas9 endonucelase, which makes the CRISPR Cas9 dependent on 4-hydroxytamoxifen.

When it comes to proteomics and broad protein profiling (and/or quantification), two main technologies come to mind: Mass Spectrometry and Antibody Arrays. Rather than opposing each other, they appear more as complementary in the chase for Biomarker discovery.

Indeed, each technique has its own advantages and limitations. Combining the two can contribute to more accurate identification of relevant biomarkers. Going one step further, biomarker identification performed by Mass Spec can be confirmed by Antibody Arrays.

Involved in many physiological processes (apoptosis, aging, cell signalling..) and numerous diseases (cardiovascular disorders, to cancer and neuro-degenerative diseases…), Mitochondria have become more and more popular as a study model in many research fields. In order to facilitate your daily work, tebu-bio provides a broad range of easy-to-use kits and assays: read on to discover the MitoTools!

I had introduced in a previous post a newly released
Allopregnanolone Immunoassay Kit (K061-H). It has actually been used in a recent study by Gao and Matsumot, published in the Journal of Ethnopharmacology (S0378-8741(18)34424-6) The authors reported significant reduction in Autism Spectrum Disorder (ASD)-like behaviors in mice treated with Kami-shoyo-san (KSS), a traditional Japanese herbal medicine. Read more in this post to see how it was used (with special thanks to Arbor Assay scientists).