Acting and reacting in life science biotechnologies
I have communicated on previous posts regarding our high density glass slide antibody arrays services.
I will focus here on another antibody microarray product line, the C-series from Raybiotech, based on a membrane antibody array technology. Similar to western blotting, they are suitable to most research laboratories for targeted protein profiling.
Over the last years, mRNA-based experimental approaches have been successfully used in a broad range of research applications (genome editing, gene replacement, vaccine, immunotherapy, cellular reprogramming…) without risk of integration into the genome of the host cell.
mRNA molecules, mimicking fully processed, capped and polyadenylated mRNA, can now be reliably produced via in vitro transcription methods while reducing innate immune stimulation. In this post, 4 tips & Tricks are presented to help produce and optimally use your mRNAs in your research and drug development projects.
Cell-based assays have become a classic way to monitor cells’ reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is Firefly Luciferase, combined or not with Renilla Luciferase. Luciferases allow simple data acquisition on a plate reader.
Nevertheless, Firefly Luciferase requires cell lysis. End-point acquisition is the only method and optimizations of the right moment is recommended. To bring more convenience, robustness and higher sensitivity, Gaussian luciferase can well be your better choice. [Read more…]
Recently, BPS Bioscience have released a new Adenosine A2A Receptor (A2AR / ADORA2A) Functional Recombinant Cell Line for screening A2AR agonists and antagonists. An excellent means to efficiently evaluate the Adenosine cell signaling pathway known to be deeply involved in all phases of inflammation and immune responses with potential interest in immunotherapies.
Due to its involvement in many cellular processes such as normal ageing, tumor suppression, chronic diseases and many more, cellular senescence has gained increasing attention since its first description over 50 years ago. In addition, understanding of cellular senescence is invaluable in the development of therapeutic targets in the fight against age-related co-morbidities, cancer and chronic disease progression.
Over-expressed in senescent cells, Senescence-associated β-galactosidase (SA-β-gal), has been widely used as a marker of cellular senescence. In order to specifically detect this biomarker directly in living cells or tissues, let’s take a look at an easy and ready-to-use assay recently developed: the SPiDER-βGal- Cellular Senescence Detection kit. [Read more…]
PBL assay specializes in the development of IFN related research Tools. One of their most appreciated assay kit by the IFN research community is the ELISA test kit for all Human IFN Alpha subtypes (41115).
Its unique features such as high sensitivity, matrix compatibility and broad detection of IFN alpha subtypes explain this popularity. [Read more…]
RayBiotech has just announced the release of a new ELISA test technology breakthrough that I am quite thrilled to share with you. If you have precious samples with low abundant target and which require high sensitivity, then this post will be of interest to you!
The RayBio® Immuno-Quantitative ELISA (IQELISA) kit is an innovative assay platform that combines the specificity and ease of use of a sandwich ELISA test with the sensitivity of real-time PCR. This results in an assay that is cutting edge and yet familiar, enabling the use of only 1/10th of the typical sample volume while providing up to 10x more sensitivity than a traditional ELISA test. The IQELISA technology is completely novel, patent pending, and has no equivalent in the market at this time. [Read more…]
The absence of mature oligodendrocytes for the repair of demyelination lesions is a critical factor in the pathology of Multiple Sclerosis (MS), and other debilitating CNS disorders of myelination. As such, regulating the maturation of oligodendrocytes and the resulting myelin production is an interesting target for potential new therapeutics.
GPR17 is an orphan G-protein-coupled receptor (GPCR) that is abundant in the CNS, and has been shown to play a key role in regulating oligodendrocyte differentiation and maturation. Unfortunately, little has been known about the exact mechanism through which GPR17 influences myelination. [Read more…]
If you work in Biology, you’ve most certainly heard of HeLa cells, as they have been around for over 60 years and are some of the most extensively used cell lines in Biomedical research. But where did these cell lines come from? [Read more…]
Focus Biomolecules produces a broad range of bioactive lipid mediators as well as many small molecule inhibitors of numerous lipid metabolic pathways for use as cellular probes. These can be supplied as individual products or in a library format (Focus Biomolecules products are available through tebu-bio in Europe). Pathways include Anandamide, Lipo-amino acid, Prostaglandin, Leukotriene, Sphingolipid, Fatty Acid, Cholesterol and more. [Read more…]
