Supplying Discovery Tools

G-LISA – a revolutionary way to study Small GTPases and Signal Transduction

21/06/2018 By Frédéric Samazan Leave a Comment

As is already known, multiple families of small GTPases (Ras, Arf and Rho families) make up the Ras GTPase superfamily. Due to their role as molecular switches in cellular signal transduction (active GTP-bound state vs inactive GDP-bound states) and in many other cellular responses (cytoskeletal reorganization, regulation of transcription, apoptosis…), these small GTPases are the subject of intense investigation to try to understand the mechanisms that regulate activation and inactivation of this proteins.

In this post, I invite you to discover a revolutionary way to evaluate and quantify the level of active small GTPases in your experimental model.

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Neurons, Astrocytes, Microglia: High Quality primary cells source

19/06/2018 By Isabelle Nobiron Leave a Comment

In the field of neurosciences, increasing research is being undertaken on neurodegenerative disorders like Parkinson’s and Alzheimer’s diseases, as well as on nerve injury and regeneration. One of the challenges researchers face is finding the appropriate cellular neuromodels, as close as possible to the in vivo environment. Primary cells are one of the answers, increasingly used in co-culture systems and 3D models. However, cells from the nervous system are not readily accessible, nor are they easy to culture.

Cell Applications Inc. provide solutions to face these challenges, ranging from highly pure, low passage ready-to-use primary cells, to stem cells and induced pluripotent stem cells (iPSC) derived from a variety of clinically relevant species. Let’s take a look at a selection of their neuron, microglia and astrocyte models in relation to their potential applications.
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Cancer immunotherapy: explore the cytokine “milieu” with Quantibody 200 Biomarker Service

18/06/2018 By Isabelle Nobiron Leave a Comment

Autologous tumour cell vaccines vs. dendritic cell vaccines: What’s best, and why? Looking at the cytokine “milieu” with the Quantibody 200 Biomarker Microarray Service helped the authors in understanding the differences in vaccine responses. [Read more…]

HDACs: Active drug targets for Immunotherapy treatment

13/06/2018 By Frédéric Samazan Leave a Comment

For many pathologies such as cancer or neurological disorders, it has been found that Histone Deacetylase (HDAC) activity is disrupted, making them promising targets for drug development. Scientists need access to a broade range of products ( Active Enzyme, inhibitors/activators, Biochemical screening assays…), to simplify their research and identification of HDAC inhibitors.

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Antibody reproducibility: what will make the difference…

12/06/2018 By Isabelle Nobiron Leave a Comment

Rockland Immunochemicals, Inc., (Rockland) provide the highest quality antibodies to the academic, biopharma, and diagnostic industries for use in basic research, assay development, preclinical and clinical studies, and bioprocessing. With facilities in Pennsylvania established since 55 years ago, Rockland manufactures products ideally suited for
integration into critical assays such as western blotting, immunohistochemistry (IHC), immunofluorescence microscopy (IF), ELISA, flow cytometry, and 2D imaging. Their antibodies show remarkable quality and reproducibility from batch to batch. Their popular anti-RFP is one example out of many.

Demonstrating Rockland’s commitment in this matter (manufacturing antibodies of high quality and reproducibility), Rockland’s CSO, Dr. Carl Ascoli, testified at the US National Academies of Sciences, Engineering and Medicine’s third of six public sessions on Reproducibility and Replicability in Science on April 18th this year. [Read more…]

Maximize metabolic activities by limiting Hepatocyte cryoinjury

07/06/2018 By Isabelle Nobiron Leave a Comment

I am following up on my series of posts based on the work done by Chris Bohl and collaborators at Sekisui Xenotech. Here, he looks at the effect of cryopreservation on pooled hepatocytes metabolic activities, and how the patented Cryostax technology can address this issue.

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Live Cell Imaging – Watch Human Osteosarcoma cells in action, stained with SiR-Tubulin probes

04/06/2018 By Frédéric Samazan Leave a Comment

As you may already know, Spirochrome have developed a broad range of Live Cell Imaging probes for the staining of DNA, Lysosome, F-Actin and Microtubules.

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New: QuicGSH3.0 – a fluorescent probe to quantify intracellular glutathione

25/05/2018 By Frédéric Samazan Leave a Comment

Glutathione (GSH) is a tri-peptide (Glutamic acid, Cystein and Glycine) present in cells, showing an antioxidant effect allowing the reduction and the removal of Reactive Oxygen Species (ROS) and Peroxide from the cell. The new QuicGSHS3.0 probe from Goryo Chemicals will allow you to easily quantify intracellular concentration of GSH by Fluorescent Live cell Imaging.

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Therapeutic Target – Ubiquitin-Proteasome system: What about tubulin?

22/05/2018 By Frédéric Samazan Leave a Comment

The ubiquitin-proteasome system (UPS) is a well-characterized protein degradation system in cells whose dysfunction is implicated in many diseases, including neurodegeneration and cancer^1,2. Major UPS components are ubiquitin (Ub), Ub ligases, Ub hydrolases (deubiquitinases [DUBs]), and the proteasome.

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Gene editing TUNR system to remix the gene expression level

21/05/2018 By Dimitri Szymczak, PhD Leave a Comment

RNAi offer >70% knock-down of the gene expression and thanks to CRISPR-CAS9 gene editing it is possible make a complete knock-out. The TUNR system comes to offer the possibility to have intermediates with high, medium and low reduction of the gene expression and with the same quality of targeting than the knock-out.