In a previous post, I introduced a selection of routine as well as innovative reagents for pluripotent stem cells (iPSC) research. Here, I’d like to present  a few more interesting products, with a focus on several manufacturers specialized in this domain: Primorigen, Stemgent and Stemcultures.

Since the discovery of reprogramming factors in 2006 and the boom of CRISPR gene editing strategies, induced pluripotent stem cells (iPSC) have emerged as new cellular models. The development of 3D cell culture technologies has also contributed to the generation of induced Pluripotent Stem Cell (iPSC) derived cells, with unique applications from patient-specific drug responses testing, to regenerative medicine. I would like to introduce in this post a selection of reagents in this domain, a combination of both routine and innovative quality reagents, that I consider as bringing something extra to your stem cell research projects.

In collaboration with our partner Ajinomoto, recognised amino-acid experts, we are launching in Europe a new Xeno-free, defined medium for Human Embryonic Stem cells and induced Pluripotent Stem cell (iPS) culture. The new StemFit^® Basic02 is a high performance single expansion medium for Stem cell and iPS cell weekend free cell culture.

Today, I’d like to invite you to take a look at a highly efficient and useful kit, which brings together all the required components you need in a complete system for culturing and transfecting human pluripotent stem cells for gene editing.

The PluriQ™ G9™ Gene Editing System includes the G9™ Maintenance Medium and G9™ VTN Recombinant (vitronectin) plate coating for culturing human induced pluripotent (iPS) or embryonic stem (hES) cells in a manner that maximizes transfection by the included EditPro™ Stem Transfection Reagent to transfect genome editing constructs.