The Wnt cell signalling pathway is a high-potential therapeutical target in cancer research. It’s deeply linked to cellular fate through modulating cell proliferation, mobility, interaction, and polarity. Wnt is not only a key player in the embryonic development but also in the homeostasis, and remodeling of adult tissues (heart, bone, neural tube, angiogenesis…) via somatic stem cell modulations and stem cell self-renewal. Abnormal Wnt signaling events are seen in various types of human carcinomas and in a variety of other cancer types.
Launched just over a year ago, SiR-Actin (Fig 1) and SiR-Tubulin (Fig 2) have been available on the market providing the most convenient tools to stain F-actin and Microtubules in living cells. In the meantime, Spirochrome have launched a third stain based on the SiR-technology – SiR-DNA (Fig 3) to stain DNA in living cells.
These stains meet the central requirements for live cell imaging tools:
The accumulation of insoluble misfolded phosphorylated tau protein in neurofibrillary tangles is one of the hallmarks of tau pathologies. Preformed fibrils generated from recombinant tau fragments have been shown to induce tau fibrilization and spreading of the pathology to interconnected brain regions in an in vivo tauopathy model. (1) Abnormal tau degradation and aggregation have been correlated with cognitive decline in Alzheimer’s disease (AD).