Study of living tissues has gained wide spread application in microscopy with the advent of fluorescent dyes and inverted microscopes dedicated to their viewing. A critical component is maintaining physiological conditions in media formulations, including pH and osmolarity, without contributing to background fluorescence. Most cells are grown in media formulations that use bicarbonate buffers, requiring the continuous presence of CO2 to maintain physiological pH. Just 15 minutes outside a CO2 incubator can change the pH from 7.4 to 8.1, and to 8.7 after an hour.

Cell reprogramming of Mouse and Human somatic cells to induced Pluripotent Stem Cells (iPSC) have opened outstanding new opportunities for biomedical research. iPSC are now becoming unique cellular models for personalized therapies and regenerative medicine.

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and influence numerous cellular functions. Together with mature RNAs and small molecules, mature miRNAs can induce forced reprogramming of somatic cells. They are considered as key regulators in stem cell development. (1, 2)

But what if you want to profile the expression of human IPS (stem cell) related miRNAs?

VEGF-ANG pathway

Angiogenesis refers to new vessel formation from the pre-existing ones. This process is essential during development, wound healing, as well as the transition of malignant tumors. VEGF family has been identified as a major contributor to angiogenesis, while families of Angiopoietin, PDGF, and FGF govern the process as well.

The most important member of VEGF family is VEGF-A, which couples with its receptor VEGFR2 to stimulate signals in endothelial cells for survival, proliferation, adhesion, migration, and tubular formation. This signaling also mediates vessel permeability and stimulates inflammatory response.

Our selection of 100% guaranteed research antibodies related to the VEGF-ANG pathway!

• ERK1 antibody: a rabbit polyclonal antibody to ERK1
• VEGFA antibody: a rabbit polyclonal antibody to VEGFA (vascular endothelial growth factor A)
• CDC42 antibody: a chicken polyclonal antibody to CDC42

Find out more antibodies specific for AKT, PI3K…

Looking for a specific intracellular pathway?

We strive to provide you regularly with new and updated pathways to support you in your research. If you are looking for a specific pathway, do not hesitate to contact us!