Nucleosomes are core particles involved in DNA packaging in eukaryotic cells. They affect gene expression levels  by regulating access of DNA to transcription factors and DNA binding proteins. Nucleosome modeling is not static and chaotic. The link between nucleosome dynamics and gene expression modulation still remains a mystery, driving Life Scientists to design clever nucleosome-based in vitro assays such as those we’ll be reviewing here.

Secrete-Pair™ dual luminescence assay kit analyzes the activities of Gaussia Luciferase (GLuc) and Secreted Alkaline Phosphatase (SEAP) as a dual-reporter system. This dual luminescence assay system includes all reagents needed for an optimal dual luminicent detection of GLuc and SEAP while allowing transfection normalization and side-by-side analysis using the same sample from the cell culture medium.

The CRISPR (Clustered, Regularly Interspaced, Short Palindromic Repeats)-Cas (CRISPR-associated) (CRISPR-Cas) system has become trendy as it is suitable for numerous applications such as gene knockouts, genome-engineering, to name but a few. In a recent Technical Bulletin, Ed Davis describes the mechanism of CRISPR-Cas for genome editing and how the recent experimental improvements improve CRISPR-Cas9 specificity while reducing off-target effects.

It’s official, CRISPR has officially reached “Breakout” status as a Google search term (see image from Google Trends). The Clustered, Regularly Interspaced, Short Palindromic Repeats (CRISPR) and RNA-guided Cas9 nucleases are an exciting new gene editing tool that allows life science researchers to directly modify the DNA of the organism they study.