Gastrointestinal (GI) primary cells represent a powerful approach for the in vitro study of the physiopathology of this unique tissue. Researchers have now identify intimate interactions between GI cells and its microbia with neurological disorders pointing out the need to access highly characterized sources of GI primary cells to design reliable and more physiologically relevant in vitro cellular models.
By mimicking in-vivo environments, 3D cell culture models appear nowadays as the best in-vitro study model to work in an in-vivo like study model and to obtain more physiologically relevant data and proof of concept as close as possible to “a clinical context” (aka the “near-human” approach).
To accompany researchers along this way, there are several innovative cell culture devices (available in Europe through tebu-bio), and more specifically a modular microfluidic platform for 3D cell culture with the capability to monitor complex biological systems dynamics in response to tuned microenvironment: the 3D Cell culture chip (Aim Biotech) [Read more…]
Following the start of our recent collaboration with Phenocell, we’re pleased to be able to provide high quality Sebocytes developed from Human induced pluripotent stem cells (iPSC). Thanks to a perfectly standardized reprogramming protocol, they display lower batch to batch variability, allowing better reproducibility and accuracy of your experimental results. [Read more…]
Understanding how mammalian cells function requires a dynamic perspective. Recent improvements in our abilities to perform fluorescence microscopy on primary cells, coupled with advances in pipelines for quantifying and extracting data, have made possible a better understanding of the temporal complexity of cell signalling pathway. Due to the heterogeneity seen in both eukaryotic and prokaryotic cell populations, study at the single cell resolution with living cells is currently the best solution to understand the dynamics between environmental conditions and cellular behaviour.
However as living cells don’t stay still, classical imaging and studying methods present some drawbacks for single cell analysis and tracking such as: [Read more…]
One of the biggest challenges as a researcher in life sciences, is to obtain proof of concept the more predictive as possible. To help you to reach your objectives, many innovative tools are being developed and new techniques are emerging. In this post, I’d like to essentially focus on two innovative cell culture devices which mimic in-vivo conditions in in-vitro systems, in fields such as 3D cell culture, Microfluidics and oriented cell culture. [Read more…]
I have to admit that I never received the Fields Medal in Mathematics. Therefore, I won’t be able to develop this equation and prove that I’m right. However, what I can prove, is that in cell culture, 2 = 5. How is this possible?
New research suggests that dendritic cells produce and release CTLA-4, which typically inhibits anticancer responses.
Cancer immunotherapy strategies have made it increasingly evident that the immune system plays an integral role in managing and destroying cancer. Nevertheless, many mechanisms of immune suppression exist that may inhibit antitumour immunity. Recently, strategies that implement antibodies directed against negative immunologic regulators have demonstrated significant success. Cytotoxic T-lymphocyte-associate protein-4 (CTLA-4) was the first immunologic checkpoint to be clinically targeted, by the cancer immunotherapeutic ipilimumab, an FDA-approved drug to treat melanoma. After T-cell activation, CTLA-4 is upregulated on the cell surface where it functions to downregulate T cell function. Ipilimumab binds to CTLA-4 on T cells, which blocks the inhibitory signals and enhances anti-cancer immune responses.
Featuring topographical resemblance to the extracellular matrix, these nanopatterned substrates provide a topographical and mechanical niche for cells to grow as if they are in vivo.
The nanopatterned surfaces influence both cellular morphology and phenotype.
For example, when used with cardiomyocytes, one can observe formation of striated sarcomeres, intercalated discs, and polarized expression of gap junction proteins such as Connexin 43 (Cx43).
Phenotypically, cells cultured on ANFS are more mature, and more physiological, so that your studies are more predictive.
If the answer is yes, I am sure you’ll be interested to learn more about WST-8 and our Cell Counting Kit-8 (CCK-8). If the answer is no, and you’ve already switched to WST-8, just have a look at our price list… you might be surprised!
Cell culture models using 2D substrates have provided important conceptual advances in understanding the biology of cells. However, cells grown on flat 2D surfaces can differ substantially from physiological environments. Animal models provide a useful tool to study biology in a physiologically relevant environment. However, animals models are expensive, time-consuming, use a significant amount of test material, and do not always provide a useful extrapolation to humans. In vitro 3D cell culture models bridge the gap between the two, allowing the study of human cells in a physiologically-relevant environment with the convenience and speed of an in vitro model. [Read more…]