Cell viability after cryopreservation is a sensitive subject as it may be dependent on the ability of mammalian cells to face the stress generated by the freeze / thaw cycles. Improving cell viability whilst maintaining optimal cellular functions and pathways has thus become a challenge. In this post, we invite you to take a look at 3 optimized reagents to improve cellular viability after cryopreservation with serum-free and protein-free CryoStor cryopreservation freeze media solutions.

When compared to 2D conventional cell culture systems, experimental data obtained with 3D cell culture models have been shown in many instances to be more biologically relevant, closer to clinical conditions (eg. predictive assays/preclinical therapeutic testing).

In this context, tebu-bio laboratories have developed a spheroid platform with a selection of downstream analysis applications such as live cell imaging, microfluidic and physioxia culture conditions or downstream multiplex biomarker quantification, among others.

If you need more physiologically relevant models for your study, but you don’t have access to the technologies or haven’t the time to spend on setting up spheroid cultures in your own laboratories, read on to see what tebu-bio laboratories can generate for you in terms of data.

Pooled human hepatocytes are a preferred test system in many drug discovery and development applications requiring intact cellular systems for in vitro testing. Intact hepatocytes contain the major hepatic drug-metabolizing enzymes and co-factors required to effectively evaluate the metabolism and potential drug-drug interactions of drug candidates.

Over the last decade, improvements in cryopreservation technologies have made using cryopreserved human hepatocytes more convenient. Pooled cryopreserved hepatocytes reduce the inter-individual differences and polymorphic distribution of liver enzymes. However, it’s crucial to carefully select a pool according to its performance but also the application it is to be used for.