Cell Sourcing – Cell Culture Technologies

3D cell culture: discover the new VitroGel™ 3D Hydrogel system

18/05/2018 By Philippe Fixe, PhD Leave a Comment

cellphy tebu-bio: in vitro assays and laboratory services for more physiologically relevant experimental data

Access tebu-bio’s cellphy offer for more physiologically relevant data and unique 3D cell culture solutions.

VitroGel™ 3D (TheWell Bioscience) is an animal origin-free hydrogel system closely mimicking the natural extracellular matrix (ECM) environment. Cells grown in this 3D hydrogel possess morphologies and cellular behaviours close to their natural state making it ideal for 3D cell culture.

Therefore, VitroGel™ 3D fits perfectly with our “cellphy” offer, providing life scientists with reagents and laboratory services mimicking the physiological environment of the Human body in vitro, for more physiologically relevant experimental data.

You can choose between two:

VitroGel™ 3D (cat. nr TWG001): an animal origin-free hydrogel system mimicking the natural ECM, ideal option for floating cell lines.
VitroGel™ 3D-RGD (cat. nr TWG002): RGD peptide modified hydrogel of VitroGel 3D promoting cell attachment and cell-matrix interactions for 3D-culture and 2D-coating (invasion/migration, chemiotaxis…). Best solution for adhesion cell lines. [Read more…]

AACR2018 – tebu-bio presents “Time-lapse analysis of drug response and invasive capacity…”

26/04/2018 By Sara Pierre Leave a Comment

This year again, we were pleased to attend the Annual meeting of the AACR (American Association for Cancer Research), which took place in Chicago, USA earlier this month.

Dora Sabino PhD of tebu-bio at the AACR2018 Poster Session

Dora Sabino Ph.D. (3D Cell Culture Project Manager, tebu-bio) presented a poster covering a physiologically relevant study on 3D colon cancer models in microfluidic chips:

“Time-lapse analysis of drug response and invasive capacity using patient-derived and CRC microfluidic 3D cancer models under oxygen controlled conditions”

Extract from "Time-lapse analysis of drug response and invasive capacity using patient-derived and CRC microfluidic 3D cancer models under oxygen controlled conditions"

Extract from tebu-bio’s poster “Time-lapse analysis of drug response and invasive capacity using patient-derived and CRC microfluidic 3D cancer models under oxygen controlled conditions”

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Download your copy of the poster here and learn more about tebu-bio’s laboratory outsourcing solutions.

Melanoma cellular line – Bridge the gap between patient and lab samples

21/03/2018 By Jean-François Têtu, PhD Leave a Comment

Tumour modelling facilitates the study of tumour growth in the tumour microenvironment (TME). Therapies that affect the tumour microenvironment are critical for advancing the fight against cancer as emerging therapies target the network of signaling pathways essential for tumour growth, Epithelial Mesenchymal Transition and metastasis (ex. Wnt/ß-Catenin pathway). Patient derived tumour models, including melanoma cell lines, are invaluable tools to study normal and malignant cells, tumour formation and drug resistance.

Rockland Immunochemicals Inc. has partnered with the Wistar Research Institute, to produce and validate a diverse panel of low passage melanoma cell lines from freshly excised metastases. More than 100 melanoma cell lines are grouped for BRAF, N-RAS, KIT, PTEN and CDK4 mutations. These pre-clinical tumour cell lines models can be used to identify the critical target genes and pathways enacted by genomic alterations and lead to more accurately prediction of the effectiveness of novel cancer therapeutics and facilitate cancer research. [Read more…]

HeLa cells: Origin of this important cell line in life science research

28/11/2017 By Xavier Faussadier Leave a Comment

If you work in Biology, you’ve most certainly heard of HeLa cells, as they have been around for over 60 years and are some of the most extensively used cell lines in Biomedical research. But where did these cell lines come from? [Read more…]

Tips for primary cell culture

15/10/2017 By Jean-François Têtu, PhD Leave a Comment

Functionality and viability of primary cells can be impaired by incorrect thawing procedures, storage or culture conditions. It’s generally admitted that applying the same protocols as for cell lines leads will lead to bad cell quality. Well, based on our experience with primary cells, here are a few tips you can follow to ensure you get the best performance.

[Read more…]

3 new types of Gastrointestinal (GI) Epithelial primary cells

04/10/2017 By Philippe Fixe, PhD Leave a Comment

Gastrointestinal (GI) primary cells represent a powerful approach for the in vitro study of the physiopathology of this unique tissue. Researchers have now identify intimate interactions between GI cells and its microbia with neurological disorders pointing out the need to access highly characterized sources of GI primary cells to design reliable and more physiologically relevant in vitro cellular models.

[Read more…]

3D cell culture Biochip – versatile for more physiological applications

15/09/2017 By Frédéric Samazan Leave a Comment

By mimicking in-vivo environments, 3D cell culture models appear nowadays as the best in-vitro study model to work in an in-vivo like study model and to obtain more physiologically relevant data and proof of concept as close as possible to “a clinical context” (aka the “near-human” approach).

3D cell culture chip – AIM Biotech tebu-bio – near-human approaches

To accompany researchers along this way, there are several innovative cell culture devices (available in Europe through tebu-bio), and more specifically a modular microfluidic platform for 3D cell culture with the capability to monitor complex biological systems dynamics in response to tuned microenvironment: the 3D Cell culture chip (Aim Biotech) [Read more…]

Pluripotent stem cells for improved reprogrammed Human Sebocytes

07/09/2017 By Frédéric Samazan Leave a Comment

Following the start of our recent collaboration with Phenocell, we’re pleased to be able to provide high quality Sebocytes developed from Human induced pluripotent stem cells (iPSC). Thanks to a perfectly standardized reprogramming protocol, they display lower batch to batch variability, allowing better reproducibility and accuracy of your experimental results. [Read more…]

Microcell arrays – obtain more accurate data from single living cells

17/07/2017 By Frédéric Samazan Leave a Comment

Understanding how mammalian cells function requires a dynamic perspective. Recent improvements in our abilities to perform fluorescence microscopy on primary cells, coupled with advances in pipelines for quantifying and extracting data, have made possible a better understanding of the temporal complexity of cell signalling pathway. Due to the heterogeneity seen in both eukaryotic and prokaryotic cell populations, study at the single cell resolution with living cells is currently the best solution to understand the dynamics between environmental conditions and cellular behaviour.

However as living cells don’t stay still, classical imaging and studying methods present some drawbacks for single cell analysis and tracking such as: [Read more…]

Human Islets for Research – Which factors affect their quality?

19/05/2017 By Jean-François Têtu, PhD Leave a Comment

The islets of Langerhans are the regions of the pancreas that contain its endocrine (i.e., hormone-producing) cells. Discovered in 1869 by German pathological anatomist Paul Langerhans, the islets of Langerhans constitute approximately 1% to 2% of the mass of the pancreas. There are about one million islets distributed throughout the pancreas of a healthy adult human. Each is separated from the surrounding pancreatic tissue by a thin fibrous connective tissue capsule. The islets of Langerhans contain beta cells, which secrete insulin, and play a significant role in diabetes.

Islets are widely used for transplantation to restore beta cell function from diabetes, offering an alternative to a complete pancreas transplantation or an artificial pancreas. Because the beta cells in the islets of Langerhans are selectively destroyed by an autoimmune process in type 1 diabetes, islet transplantation is a means of restoring physiological beta cell function in patients with type 1 diabetes.

Human Islets for Research (HIR)® are primary human islets processed from organ donor pancreases that have been approved for research but not for clinical transplantation of either the pancreas or the isolated islets. HIR® are obtained in a proprietary process of pancreas digestion and islet purification that results in uniformly high quality HIR® for delivery to diabetes investigators. Quality Control (QC) testing is routinely performed prior to release to assure uniform quality and function of these islets available for research. [Read more…]