Actin filaments are essential for cytoskeleton functions (cell morphology, endocytosis and trafficking, contractility, motility…). In eukaryotic cells, polymerization and depolymerization of actin filaments together with their organization in higher magnitude actin-based superstructures and their complex dynamic properties are regulated by Actin-Binding Proteins (ABPs). Here, let’s review the ABPs recently released for cytoskeleton molecular modeling studies.
The Janus kinase (JAK) / Signal Transducer and Activator of Transcription (STAT) pathway a key regulator of cellular proliferation, differentiation, migration, apoptosis and survival. Numerous cytokines, growth factors and Interferons interact with the JAK/STAT signaling pathway which in turn regulates many biological processes, especially in hematopoietic development and immune responses.
NF-κB (Nuclear Factor κ-light-chain-enhancer of activated B cells) is a protein complex that controls the transcription of DNA. Find the updated version of the NF-κB cell signaling pathway.
In a recent paper published in PNAS, Wang et al. show that the inhibition of the deubiquitinase USP9x by the small molecule WP-1130 is able to induce degradation of the transcription factor E-twenty-six related gene (ERG).
Protein phosphorylation analysis is more and more used for cell signaling studies. This post-translational modification regulates the function of numerous proteins in both physiological and disease-related conditions. Phospho-proteins can be easily monitored by using phospho-specific antibodies in various types of immuno-assays (Western blot, antibody array, phospho-ELISA…). However, data generated is reliable only if used from cell or tissue lysates that effectively reflect the phosphorylation state of initial samples. For this, optimal cell lysis buffers preserving phosphorylation status and protein stabilty are crucial. [Read more…]
The transition from non-invasive phenotype to invasive phenotype of tumor cells marks the switch from a benign tumor to a more malignant form of cancer. Understanding the mechanisms underlying this hallmark event, which enables tumor cells to invade through Extracellular matrix, is critical for discovering pathways and new targets to develop anti-metastatic strategies. The discovery of anti-metastatic agents that inhibit cancer cell motility has been hindered by a dearth of cell motility assays that are compatible with high–throughput screening. [Read more…]
Cell reprogramming of Mouse and Human somatic cells to induced Pluripotent Stem Cells (iPSC) have opened outstanding new opportunities for biomedical research. iPSC are now becoming unique cellular models for personalized therapies and regenerative medicine.
Low oxygen tension, or Hypoxia, regulates numerous cellular and tissular functions. In cancer research, hypoxia is a key regulator of tumor development, aggressiveness and therapy resistance by acting on malignant cells and their microenvironment. Hypoxia is also involved in age-related diseases and acts through intracellular and intercellular cascade of events (exosomes, paracrine loops, angiogenesis…) (1-2). [Read more…]