In a few forthcoming posts, I’d like to share several short articles published by Dr Chris Bohl of Sekisui Xenotech, a technical expert in the field of ADME-Tox tools and applications. I feel they may be of special interest to researchers working in the field of ADME-Tox studies.

Today, we’ll take a look at compounds that exhibit high metabolic stability in hepatocytes and subcellular fractions (S9, microsomes and cytosol), as these can be a challenge for ADME scientists.

Sekisui Xenotech’s Research BioBank provides you with normal and diseased human liver tissue samples for scientific investigation. Tissues are collected with the initial intent for transplant, thus making them perfect candidates to advance basic medical science and contribute to the development of new treatments.

The care taken to preserve viability of the tissue intended for transplantation distinguishes these Research Biobank specimens from samples collected at a typical post-mortem, usually compromised by several hours of a warm ischemia. So what are the other benefits of the biobank?

Sekisui XenoTech now offers human liver tissue microarrays (TMA) and a microsomal pool for studying and developing new treatments for fatty liver disease (FLD). The microsomes and arrays feature non-alcoholic steatohepatitis (NASH) and other liver tissue from the company’s Research Biobank . The initiative is part of Sekisui XenoTech’s ongoing commitment to furthering knowledge of hepatic diseases.

“Fatty liver disease is a highly pervasive condition that has a very significant impact on the health of modern society. We are supporting hepatology researchers with these collections of high-quality human tissues to advance basic knowledge of FLD and to develop new treatments for the illness,” explained Maciej Czerwinski, Ph.D., Sekisui XenoTech Director of Consulting who led the effort to establish the company’s research Biobank and develop the microarrays.

Isolation of Subcelluar Fractions for use in ADME studies – Sekisui Xenotech and tebu-bio

Over the years, Sekisui XenoTech has built up a strong expertise in preparing subcellular fractions for ADME applications.

There is a list of the fractions readily available for prompt delivery across Europe. However, the researcher’s specific needs in terms of species model, characterization or tissue for a particular metabolism study may not be met by the already available catalogue products. For this reason, the existing list is continuously expanding over the years in response to new demands.

Much experience has been gained by Sekisui Xenotech through interesting requests for custom tissue preparations from researchers. Here, I’d like to share some comments from the scientists at Xenotech involved in these preparations, to give you some insight in their customization capacities.