Wnt4 and Rspo1 to promote mammary stem cell self-renewal


WNT4 antibody detects WNT4 protein by western blot analysis. Non-transfected (-) and Wnt4-transfected (+, including V5-tag) 293T whole cell extracts (100 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 antibody (Cat. No. 210GTX101085) at a dilution of 1:2500. The V5 was used as internal control (Cat. No. 210GTX117997, 1:2500) shown at the bottom panel.

The behavior of adult mammary stem cells (MaSCs) is precisely controlled by the activities of hormones and local factors, though the underlying mechanisms remain obscure. A recent report by Cai et al in Genes & Development illustrates the dynamic interaction between systemic ovarian hormones, Wnt signaling, and the Wnt agonist R-spondin1 (Rspo1) to promote MaSC self-renewal.

In response to estradiol and progesterone, R-spondin1 and Wnt4, but not Wnt7B, act as niche factors to drive MaSC regeneration, with Wnt4 acting through the canonical Wnt/β-catenin signaling pathway. This work establishes a clear mechanistic link between locally acting Wnt signals and the systemic hormone growth response of MaSCs, unveiling the intriguing concept that hormones induce a collaborative local niche environment for stem cells.

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