Breast Cancer – Popular validated primary antibodies for research and biomarker discovery

Breast Cancer

Use of validated antibodies is primordial for Breast Cancer research and related biomarker discovery. Here, we’ll review the main biomarkers involved in this disease and introduce the most popular validated primary antibodies used by our clients.

Most breast cancers start in the cells lining the breast ducts or lobules (ductal/lobular cancers) mainly in women, with 89 cases per 100 000 (see “Anti-MUC1 Antibody in Nipple Aspirate Fluids Correlates with Tumor Aggressiveness in Breast Cancer: A Feasibility Study” by Menekse E. et al – Disease Markers
Volume 2015 (2015), Article ID 179689).

Sporadic or non-hereditary cancers represent 85-90% of breast cancers and usually have unidentified environmental origins (only 5-10% have an inherited genetic origin). Some of these breast cancers can be attributed to life style choices (alcohol, trans-fats, obesity, lack of exercise) or gynecological factors (late first pregnancy, not breast-feeding, etc.).

Anti-c-erbB2 Monoclonal Antibody (Rabbit) was used to detect c-erbB2 in human breast tumor tissue. Tissue was formalin-fixed and paraffin embedded. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min. The primary antibody (cat. nr 900-C01-B37-C) was diluted 1:100 and reacted with tissue for 30 min at RT.

Anti-c-erbB2 Monoclonal Antibody (Rabbit) was used to detect c-erbB2 in human breast tumor tissue. Tissue was formalin-fixed and paraffin embedded. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min. The primary antibody (cat. nr 900-C01-B37-C) was diluted 1:100 and reacted with tissue for 30 min at Room Temperature.

A significant proportion of sporadic breast cancers are induced by hormonal treatment in women having a predisposition to this type of cancer.

If detected at an early stage, breast cancer can be cured in 9 cases out of 10. Nevertheless, one of the difficulties of curing this disease is to identify the biological characteristics of each patient’s tumor.

The main breast cancer biomarkers

The usual first elements to further characterize breast cancer are the Human Epidermal Growth Factor Receptor-2 (c-erbB-2 / HER2) biomarker expression level (see HER2 Status | and the Estrogen & Progesterone status levels (see Breast Cancer Hormone Receptor Status |

HER2 is a protein (encoded by the HER2/Neu gene) naturally present in the organism. This cancer biomarker is a transmembrane receptor involved in the regulation of cell proliferation. In some cases of breast cancer, HER2 is overexpressed on the cell surface, resulting in an uncontrolled growth and division of the cells. This is now systematically analyzed in Cancer Centers to know if patients can benefit from endocrine therapy and anti-HER2 targeted therapy.

The Ki67 biomarker is also widely used in breast cancer prognosis to evaluate the proliferation level of a tumor. Ki67 is a protein only present in the nucleus of dividing cells and analyzing its proportion, informs about the proliferation level of the tumor. More proliferative tumors grow faster and generally associate with a poorer prognosis (see “Assessment of Ki67 in Breast Cancer: Recommendations from the International Ki67 in Breast Cancer Working Group” by Dowsett M. et al. – J Natl Cancer Inst. 2011 Nov 16; 103(22): 1656–1664)

In parallel, the detection of well-known markers such as Mucin 1 (MUC1) is assessed too ( see “MUC1-C Oncoprotein as a Target in Breast Cancer; Activation of Signaling Pathways and Therapeutic Approaches” by Donald W. Kufe – Oncogene. 2013 Feb 28; 32(9): 1073–1081). This molecule is localized on the cell surface in 70% of the deadliest types of cancer including some particular breast cancers. Indeed, MUC1 is present in 90% of so-called triple-negative breast cancers, insensitive to hormone treatment.

Research projects have also established connections between mutations in the BRCA1 and BRCA2 genes and some familial breast & ovarian carcinomas (see “BRCA1 and BRCA2 mutations account for a large proportion of ovarian carcinoma cases” by Pal T – Cancer. 2005 Dec 15;104(12):2807-16).

BRCA1 and BRCA2 are known genes of susceptibility to breast cancer and ovarian cancers. These genes are described as “high penetrance” because they are associated with an elevated risk of developing breast and ovarian cancers in women who have mutations in these genes. In the general population, there is approximately 1 person out of 1,000 that has mutations in the BRCA1 and/or BRCA2 genes.

There is an ongoing interest in improved early detection and prognosis, several multianalyte tests have been proposed for predicting outcome in patients with newly diagnosed primary invasive breast cancer, like MammaPrint or Oncotype DX. Also, recent studies have high-lighted RARA (Retinoic Acid Receptor α), aromatase expression or osteopontin as potential highly reliable biomarkers.

Validated primary antibodies for biomarker and breast cancer research

In order to be able to identify and validate new cancer biomarkers, validated primary antibodies are required in the early stages of the pre-clinical research programs. Here are the most popular antibodies used by scientists involved in breast cancer research together with their validated applications.

  • Anti BRCA1 cat. nr BS6423 (source: Bioworld-tebu-bio) for WB, IHC, and IF
  • Anti BRCA2 Cat. nr C10682 (source: Assay Biotech-tebu-bio) for IHC and ELISA
  • Anti Aromatase Cat. number cat. nr PA1489 (source: BosterBio-tebu-bio) for WB, IHC (P)
  • Anti EGFR phospho Y1197 cat. nr 600-401-928 (source: Rockland-tebu-bio) for ELISA, WB and IHC
  • Anti ERBB2/HER2 cat. nr H00002064-M05 (source: Abnova-tebu-bio) for ELISA, IHC (P), in situ PLA, WB
  • Anti-Estrogen Receptor cat. nr 900-C01-B35 (source: Rockland-tebu-bio) for IHC and WB
  • Anti GSK3B cat. nr H00002932-M04 (source: Abnova-tebu-bio) for ELISA, IHC (P), in situ PLA, WB
  • Anti Ki-67 (clone SP6) Cat. nr 900-C01-B38 (source: Rockland-tebu-bio) for ELISA, WB and IHC
  • Anti KIT cat. nr H00003815-M07 (source: Abnova-tebu-bio) for WB and ELISA
  • Anti MUC1 (clone 3B9) cat. nr H00004582-M01 (source: Abnova-tebu-bio) for WB and ELISA
  • Anti Osteopontin Cat. nr C0284 (source Assay Biotech-tebu-bio) for WB and ELISA
  • Anti PIK3CA Cat. nr H00005290-M01 (source: Abnova-tebu-bio) for in situ PLA and ELISA
  • Anti Progesterone Receptor (clone 5D10) cat. nr H00005241-M07 (source: Abnova-tebu-bio) for WB, IHC, and IF
  • Anti PTEN cat. nr H00005728-M01 (source: Abnova-tebu-bio) for ELISA, in situ PLA, and WB
  • Anti RARA Cat. nr BS7672 (source: Bioworld-tebu-bio) for WB, IHC and IF
  • Anti WNT5A cat. nr 200-401-I36 (source: Rockland-tebu-bio) for WB

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Looking for more primary antibodies for cancer research?

tebu-bio offers a large selection of high quality primary research antibodies and guarantees them for their recommended applications. Leave a message here if you want to contact tebu-bio’s antibody experts to find the most appropriate antibody, protocol, positive controls… best suited to your needs.

Additional information regarding the use of primary antibodies

Thanks to Xavier Faussadier for his help in the writing of this post dedicated to Breast Cancer research antibodies.

Written by Philippe Fixe, PhD
Philippe Fixe is a former Marketing Manager at tebu-bio, passionate about innovation and R&D in Life sciences, Biotechnology, Medical research, Drug discovery, and also a keen photographer.