Antibody generation raises a number of questions… what is the difference between their classes, forms and types? And how do recombinant monoclonal antibodies (rAbs) overcome the drawbacks of classical monoclonal antibodies (mAbs)?

Monoclonal antibodies are ubiquitous in biomedical research and medicine. They are used to fight, diagnose and research disease and to develop and test new drugs. The antibodies are divided in 5 classes or isotypes, several subtypes and forms and can be generated in vivo or in vitro.

In this short article, my aim is to define what an antibody is, to highlight the differences between a hybridoma monoclonal antibody and a recombinant monoclonal antibody and to point out how rAbs bring solutions to the classical drawbacks of mAbs.

Recombinant protein expression and purification for therapeutic development or for in vitro studies can sometimes be a real challenge. It requires huge investments in both time and cost in order to obtain high yields of pure and active recombinant proteins. My aim in writing this post, is to give you a simple guide to easily establish optimal conditions for recombinant protein expression and production in E. Coli, of problematic unstable proteins of interest.

Mass spectrometry is a technology which is widely used in most scientific disciplines that require accurate and precise measurement of elemental and molecular components. Its use in the pharmaceutical sector is often associated with the Drug Discovery and Development process. The primordial step to analyze a sample is to perform a sample treatment to enable its study. It really requires know-how to obtain good quality results. The following tips and tricks can help you to rapidly select a protein purification strategy and to anticipate some possible problems.

Decidedly, producing and purifying a protein is a form of art.

Indeed, each recombinant protein (rec. protein) to be produced in vitro has specific characteristics and singularities that make its production and purification most definitely challenging. In this post, I’d like to share and review a few tips and basics for optimal design of experiment when producing functional recombinant proteins and working on protein purification.