Frédéric Samazan

Cell signalling – Discover G-LISA to study Small GTPases

21/06/2018 By Frédéric Samazan Leave a Comment

As is already known, multiple families of small GTPases (Ras, Arf and Rho families) make up the Ras GTPase superfamily. Due to their role as molecular switches in cell signalling (active GTP-bound state vs inactive GDP-bound states) and in many other cellular responses (cytoskeletal reorganization, regulation of transcription, apoptosis…), these small GTPases are the subject of intense investigation to try to understand the mechanisms that regulate activation and inactivation of this proteins.

In this post, I invite you to discover a revolutionary way to study these cell signalling events by quantifying the level of active small GTPases in your experimental model.

[Read more…]

HDACs: Active drug targets for Immunotherapy treatment

13/06/2018 By Frédéric Samazan Leave a Comment

For many pathologies such as cancer or neurological disorders, it has been found that Histone Deacetylase (HDAC) activity is disrupted, making them promising targets for drug development. Scientists need access to a broade range of products ( Active Enzyme, inhibitors/activators, Biochemical screening assays…), to simplify their research and identification of HDAC inhibitors.

[Read more…]

Live Cell Imaging – Watch Human Osteosarcoma cells in action, stained with SiR-Tubulin probes

04/06/2018 By Frédéric Samazan Leave a Comment

As you may already know, Spirochrome have developed a broad range of Live Cell Imaging probes for the staining of DNA, Lysosome, F-Actin and Microtubules.

[Read more…]

New: QuicGSH3.0 – a fluorescent probe to quantify intracellular glutathione

25/05/2018 By Frédéric Samazan Leave a Comment

Glutathione (GSH) is a tri-peptide (Glutamic acid, Cystein and Glycine) present in cells, showing an antioxidant effect allowing the reduction and the removal of Reactive Oxygen Species (ROS) and Peroxide from the cell. The new QuicGSHS3.0 probe from Goryo Chemicals will allow you to easily quantify intracellular concentration of GSH by Fluorescent Live cell Imaging.

[Read more…]

Therapeutic Target – Ubiquitin-Proteasome system: What about tubulin?

22/05/2018 By Frédéric Samazan Leave a Comment

The ubiquitin-proteasome system (UPS) is a well-characterized protein degradation system in cells whose dysfunction is implicated in many diseases, including neurodegeneration and cancer^1,2. Major UPS components are ubiquitin (Ub), Ub ligases, Ub hydrolases (deubiquitinases [DUBs]), and the proteasome.

[Read more…]

AIM Biochips – 3D cell culture platform for Immunotherapy and T Cell therapy studies

18/05/2018 By Frédéric Samazan Leave a Comment

As mentioned in a previous post the DAX Biochips from AIM Biotech are an innovative and versatile microfluidic platform, allowing researchers to more easily develop 3D cell culture models, to work as close as possible to what occurs in the human body, but under in-vitro conditions.

Due to its innovative structure (as seen in this general AIM Chips flyer), a broad range of applications have already been validated on this model (Cellular Migration-Invasion Analysis / Angiogenesis studies / Metstasis Modeling System), but what’s new is that now Immunotherapy and T-Cell Therapy studies can be performed on this technology.

[Read more…]

Cell signalling studies: Tips for selecting the best Small G Protein activation assay

25/04/2018 By Frédéric Samazan Leave a Comment

Small GTP-binding proteins such as Rho and Ras GTPase family members are involved in regulating cell signalling pathways and impact a wide range of cellular processes, functions, and morphology. In this post, you’ll discover two types of Small G-protein Activation Assays to easily measure the GTP-bound form of your protein of interest, as well as some tips for choosing between both solutions and finding the assays that fit best with your cell signalling experiments.

[Read more…]

Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection

17/04/2018 By Frédéric Samazan Leave a Comment

Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.

[Read more…]

How to make Post Translational Modification characterization easier

10/04/2018 By Frédéric Samazan Leave a Comment

Signal-Seeker assays (Cytoskeleton Inc.) provide researchers with the most comprehensive reagents enabling highly sensitive and specific detection of the key Post Translational Modifications (PTMs) of your Protein of Interest.

In this post, I invite you to look at experimental results obtained with these unique assays for the characterization of Tyrosine Phosphorylation, Ubiquitination, SUMOylation and Acetylation of endogenous proteins. [Read more…]

Quantify ExtraCellular Matrix components – 5 popular kits you need to know

28/02/2018 By Frédéric Samazan Leave a Comment

Sircol collagen assay standards – Biocolor at tebu-bio

In living organisms, ExtraCellular Matrix (ECM) builds up a complex and dynamic network of molecules, playing a major role in structural support, adhesion, movement and cellular regulation. Major components of the ECM (Collagen, Proteoglycans, Elastin, Hyaluronan) are key elements studied in the Pharmaceutical and Cosmetology fields due to their strong involvement in tissue regeneration, skin repair, wound healing, ageing diseases, surgery, inflammation, and also tumour development.

Boost your ECM studies with these 5 kits!

To help you in monitoring this component, let’s take a look at five robust and easy-to-use, in vitro assays, with colorimetric read-outs for easier daily use in your research.