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Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
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  • Meet the authors
Cell Biology and Signalling

Lipid-Ligand interaction tools: lipids are not scary intermediates anymore

24/06/2019 by Paola Vecino, PhD 6 Comments

The first time a cell biologist discovers that there might be a lipid involved in their favourite pathway, it’s a bit like finding a wall across the road (just the same situation as when a lipidomist finds a protein in their way…).

Due to the different nature of lipids, most cell biologists fear having to face this situation along their way. The good news is that there are some simple methods to help overcome this obstacle, and make the studyof lipids in a given pathway easier. Here, I’d like to go over some analytical and preparative tools for lipid-ligand interaction studies.

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Cell Biology and Signalling

Synthetic Glycolipids for in vitro immune response analysis

21/01/2016 by Paola Vecino, PhD No Comments
Infectious diseases, Immunity, Antibiotics

Efficient immune response against an infectious agent is organised according to a “two-legged” pattern. In order to give the immune system enough time to fine-tune its weapons against the invader (whilst maintaining the threat at bay), the first line of defence mobilises ancestral effectors during the first hours of the invasion. These “innate” defences aim at stimulating a dormant, but far more specialised and potent, second line of defence: the adaptive immune system.

That’s quite an elaborate chain of reactions… so what role do glycolipids play in immune response?

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Supplying Discovery Tools

Adenovirus: weapons of mass transduction for gene delivery

24/02/2015 by Paola Vecino, PhD 2 Comments

Adenovirus (AV) are non-enveloped and non-integrative icosaedral viruses. Recombinant AV (genetically modified with respect to the wild-type in order to lose their capacity of replication) are widely used for their high infectivity (almost 100% in almost all mammalian cell types) their high level of expression of exogenous protein (up to 20% of total host protein expression), their episomal maintenance (non-integrative, non-genome disruptive ) and because they are easy-to-use in vitro and in vivo.

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Gene Expression - Molecular Biology

Next generation of lentiviral particles

12/02/2015 by Paola Vecino, PhD No Comments

Lentiviruses are robust tools for cell line engineering and development. As opposed to models that only infect dividing cells, lentiviral vectors are ideal for long term gene delivery into dividing and quiescent cells. This post is aimed at introducing the newest evolutions of lentiviral experimental approaches: the 3rd generation lentiviral plasmids.

Over the last decade, Lentivirus progressively became a research tool for stable cell line engineering and production. What makes them so attractive?

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