The Ras-Raf-MEK-ERK Pathway is an important cell signalling route with many implications for cancer biology and therapeutic development. Dis-regulation of some of this pathway’s proteins expression and phosphorylation status are observed in about one-third of all human cancers. Access to specific tools to study this pathway is essential to a better understanding of its role in cancer for novel drug development. If you are working on this topic, you’ll be interested in taking a look at the range of reagents on offer for a wide variety of applications.
If you are looking for enzymatic in vitro tools for drug metabolism, reaction phenotyping, metabolite generation, using pig as model, you may find these newly released Pig Bactosomes from Cypex of interest.
Bactosomes are recombinant enzymes expressed in E. coli with a patented expression system that enables expression of drug metabolising enzymes without the need for large modifications to be made to the proteins. They demonstrate excellent batch-to-batch consistency and robust activity levels. Each batch is characterized for linearity with time and CYP concentration, Km and Vmax. [Read more…]
Metabolite characterization can be a lengthy process making your in vitro drug testing time consuming and expensive. Whether you are working on phase I or phase II metabolic enzymatic reactions, take a look at these BMO kits which can help you speed up this characterisation process.
- Perform the primary screen and select the desired metabolite wells (XTHCK1001-01)
- Perform the optimization and identify the best production conditions
- Scale-up and isolate the metabolite
A single-domain antibody (sdAb, also called Nanobody) is an antibody fragment consisting of a single monomeric variable antibody domain. Like a whole antibody, it is able to bind selectively to a specific antigen. With a molecular weight of only 12–15 kDA, single-domain antibodies are much smaller than common antibodies (150–160 kDa). They have been shown to be just as specific as a regular antibody and in some cases more robust. They are being researched for multiple pharmaceutical applications in in vivo imaging and targeted therapy. eg. the fusion of a fluorescent protein to a single-domain antibody can be used to trace targets in different compartments of living cells. They can therefore increase the possibilities of live cell microscopy and will enable novel functional studies. [Read more…]
Sekisui XenoTech has just been issued U.S. Patent No. 9,642,355 for the “cryopreservation of cells and subcellular fractions,” specifically related to Sekisui XenoTech’s CryostaX® test systems. This hepatocyte cryopreservation method was invented by Maciej Czerwinski, Ph.D., Director of Consulting for Sekisui XenoTech. [Read more…]
The mitogen-activated protein kinase (MAPK) signaling pathway is activated by a number of extra and intracellular stimuli including cytokines, growth factors, and hormones as well as stressors such as oxidative and ER stress. This pathways plays a key role in the regulation of many cellular processes including proliferation, differentiation, the stress response, motility, growth, differentiation, survival, and death. Abnormal MAPK signaling may contribute to increased or uncontrolled cell proliferation and/or resistance to apoptosis. To study this complex pathway, several tools are available, from the pathway specific arrays for an initial screen, to phospho-specific ELISA tests for individual target validation. This post aims at helping you to easily identify tools to explore this pathway in your samples (from arrays to phospho-ELISAs). However, I could not start without showing you once more one of these eye-catching illustrations of cell signalling pathways. I’ll let you explore it to dig out the MAPK protein cascade among all of them (a kind of Where’s Wally for the researcher !).
Since the discovery of reprogramming factors in 2006 and the boom of CRISPR gene editing strategies, induced pluripotent stem cells (iPSC) have emerged as new cellular models. The development of 3D cell culture technologies has also contributed to the generation of induced Pluripotent Stem Cell (iPSC) derived cells, with unique applications from patient-specific drug responses testing, to regenerative medicine. I would like to introduce in this post a selection of reagents in this domain, a combination of both routine and innovative quality reagents, that I consider as bringing something extra to your stem cell research projects.
In a previous post dedicated to Quantitative arrays (Quantibody), I introduced our L-Series aimed at a broad one shot profiling of up to 1000 markers at once. This relative quantitation technology allows you to perform a first screen of your samples of interest versus a control, before you go on to targeted profiling using either pathway specific arrays, or a custom array including the targets of interest identified with the initial L-series screen. [Read more…]
On your journey to Biomarker profiling, you will reach the point where you need to quantify the proteins of interest identified along the way (eg. validation of semi-quantitative array results; biomarker discovery with an initial hunch on which pathway is involved).
At this stage, you will have narrowed down the number of targets you want to look at. However, the use of ELISAs is likely to be still too costly, too time consuming, and may require too much sample volume. [Read more…]
Progesterone derived Allopregnanolone (ALLO) is an extensively studied neurosteroid that has been shown to be involved in neurodegenerative diseases, including Alzheimer’s, Parkinson’s disease, and multiple sclerosis. It is also linked to neuroinflammatory processes through a beneficial action on pro-inflammatory cytokines.
Allopregnanolone detection kits based on polyclonal antibodies were already available through Arbor Assays (K044-H and K044-C), a specialist of immunassay kits, with a special focus on inflammation and reproduction targets.
A new Allopregnanolone EIA kit has just been released, based on a monoclonal antibody (K061-H1). In addition to the supply stability offered by the monoclonal antibodies, this new kit is more sensitive than the original one, it requires smaller sample volumes and provides increased signal. It also has a more favorable cross reactivity profile: [Read more…]