Following up on my series of posts based on Dr Chris Bohl’s work at Sekisui Xenotech, in this post I invite you to take a look at the work he has published, together with Dr Christopher Seib, Maciej Czerwinski, Zell Woodworth and David Buckley, Ph.D., illustrating the characterization of isolated human liver lysosomes, and validating them as test systems for in vitro assessment of catabolic stability of biologics drugs entering the cell by the endosomal–lysosomal pathway.
iPSC Research: specialized manufacturers for quality reagents
In a previous post, I introduced a selection of routine as well as innovative reagents for pluripotent stem cells (iPSC) research. Here, I’d like to present a few more interesting products, with a focus on several manufacturers specialized in this domain: Primorigen, Stemgent and Stemcultures.
Protein multiplex profiling as simple as western blot
In one of my previous posts, I wrote about high density glass slide antibody arrays services.
Here, I’d like to focus on another antibody microarray product line, the C-series from Raybiotech, based on a membrane antibody array technology. Similar to western blotting – therefore very easy to process – they are suitable to most research laboratories for targeted protein profiling.
What to do with Microsome Stable, Low-Turnover Compounds
In a few forthcoming posts, I’d like to share several short articles published by Dr Chris Bohl of Sekisui Xenotech, a technical expert in the field of ADME-Tox tools and applications. I feel they may be of special interest to researchers working in the field of ADME-Tox studies.
Today, we’ll take a look at compounds that exhibit high metabolic stability in hepatocytes and subcellular fractions (S9, microsomes and cytosol), as these can be a challenge for ADME scientists. [Read more…]
Liver tissue BioBank: High quality specimens for liver disease research
Sekisui Xenotech’s Research BioBank provides you with normal and diseased human liver tissue samples for scientific investigation. Tissues are collected with the initial intent for transplant, thus making them perfect candidates to advance basic medical science and contribute to the development of new treatments.
The care taken to preserve viability of the tissue intended for transplantation distinguishes these Research Biobank specimens from samples collected at a typical post-mortem, usually compromised by several hours of a warm ischemia. So what are the other benefits of the biobank? [Read more…]
Fatty Liver Disease Research: new Tissue Arrays and microsome pool
Sekisui XenoTech now offers human liver tissue microarrays (TMA) and a microsomal pool for studying and developing new treatments for fatty liver disease (FLD). The microsomes and arrays feature non-alcoholic steatohepatitis (NASH) and other liver tissue from the company’s Research Biobank . The initiative is part of Sekisui XenoTech’s ongoing commitment to furthering knowledge of hepatic diseases.
“Fatty liver disease is a highly pervasive condition that has a very significant impact on the health of modern society. We are supporting hepatology researchers with these collections of high-quality human tissues to advance basic knowledge of FLD and to develop new treatments for the illness,” explained Maciej Czerwinski, Ph.D., Sekisui XenoTech Director of Consulting who led the effort to establish the company’s research Biobank and develop the microarrays. [Read more…]
Subcellular fractions for ADME studies: broad custom capacities with strong expertise
Isolation of Subcelluar Fractions for use in ADME studies – Sekisui Xenotech and tebu-bio
Over the years, Sekisui XenoTech has built up a strong expertise in preparing subcellular fractions for ADME applications.
There is a list of the fractions readily available for prompt delivery across Europe. However, the researcher’s specific needs in terms of species model, characterization or tissue for a particular metabolism study may not be met by the already available catalogue products. For this reason, the existing list is continuously expanding over the years in response to new demands.
Much experience has been gained by Sekisui Xenotech through interesting requests for custom tissue preparations from researchers. Here, I’d like to share some comments from the scientists at Xenotech involved in these preparations, to give you some insight in their customization capacities. [Read more…]
Recombinant CYP Bactosomes: versatile formats for many ADME applications
Recombinant CYPs are commonly used in ADME-Tox studies as in vitro tools, mostly for reaction phenotyping, enzyme inhibition screening, clearance and metabolite ID.
“Research has shown that rCYPs expressed in E. coli (Bactosomes) showed greater enzymatic activity compared with the equivalent enzymes expressed in insect cells (Supersomes). Similarly, most Bactosomes show greater turnover numbers when compared with the equivalent Supersomes. These results demonstrate that human rCYPs expressed in E. coli are frequently more active than the corresponding rCYPs expressed in insect cells» says Michael Voice from CYPEX. [Read more…]
New – 4 Unique IFN Neutralizing reagents
PBL Assay Science, the specialists of Interferon related quality reagents, have just released 4 new unique products to be used in neutralization assays. I am pleased to introduce them in this post, in which you’ll discover examples of titration data, as well as reactivity and cross-reactivity information. [Read more…]
Human IFN ELISA test: unrivaled sensitivity and global IFN alpha detection
PBL assay specializes in the development of IFN related research Tools. One of their most appreciated assay kit by the IFN research community is the ELISA test kit for all Human IFN Alpha subtypes (41115).
Its unique features such as high sensitivity, matrix compatibility and broad detection of IFN alpha subtypes explain this popularity. [Read more…]