The sensitivity and specificity of the primary and secondary antibodies used together with the IHC procedure used, are critical to avoid biased results.Â Several factors can cause false-positive or false-negative data, so theyÂ should all be verified as much as possible for each experimental set-up.
- Detection of the antigen of interest by the primary antibody
- Detection of the primary antibody by secondary antibodies
- Tissue preparation
Here, let’s look atÂ 3 tips that will be of help to improve your IHC data.
Following our series of posts on tumour immunity, I’d like to mention anÂ articleÂ published by researchers from theÂ Ben-Gurion University of the Negev (Israel). The authorsÂ investigated the negative correlation between caffeine consumption and incidence of tumours. Or to say it more simply, intake of caffeine favours the immune system fighting tumours. (1)