New – 4 Unique IFN Neutralizing reagents

PBL Assay Science, the specialists of Interferon related quality reagents, have just released 4 new unique products to be used in neutralization assays. I am pleased to introduce them in this post, in which you’ll discover examples of titration data, as well as reactivity and cross-reactivity information.

#1- Human Type I IFN Neutralizing antibody mixture

  • Mixture of monoclonal and polyclonal antibodies directed against human Type 1 Interferons (IFNs) and Type 1 Receptor Subunit 2 (IFNAR2) (cat. nr 39000-1)
  • Effectively neutralizes biological activity of human IFN-alpha, IFN-beta, IFN-omega, IFN-kappa, and IFN-epsilon
  • Improves neutralization of multiple Type I IFNs often found in complex samples by employing a broadly neutralizing custom antibody cocktail
  • No cross reactivity observed with the following human

Representative neutralizing curves are shown below:

Human Type I IFN Neutralizing antibody mixture (cat. nr. 39000-1) - PBL / tebu-bio

Representative neutralize curve obtained with the Human Type I IFN Neutralizing antibody mixture (cat. nr. 39000-1). Source: PBL / tebu-bio

In this assay, A549 cells were incubated with a fixed amount of neutralizing antibody mixture and varying IFN concentrations, followed by challenging with encephalomyocarditis virus (EMCV).

#2- Human IFN Lambda 2/IL-29 Neutralizing antibody

  • Mouse monoclonal antibody against Human IL-28A/IFNL2 (cat. nr 21720) – Clone MMHL-2
  • Neutralizes human IL-28A/IFNL2 bioactivity
  • ~3% and 8% cross-reactivity is observed with human IFNL1 and human IFNL3, respectively

Representative Neutralizing curves shown below:

Representative curve obtained with the Human IFN Lambda 2/IL-29 Neutralizing antibody (cat. nr. 21720). Source: PBL / tebu-bio.

Representative curve obtained with the Human IFN Lambda 2/IL-29 Neutralizing antibody (cat. nr. 21720). Source: PBL / tebu-bio.

IFN Lambda 2 antibody tiration against human IFN Lambda in A549/EMCV antiviral assay.

#3- Neutralizing C12R Mouse IFN Alpha Decoy Receptor

  • Viral C12R IFN Antagonist selectively neutralizes all mouse IFN alpha subtypes (cat. nr 12185-1)
  • No cross-reactivity with mouse IFN beta
  • Also neutralizes all human IFN alpha subtypes, human IFN beta, human IFN omega, rat IFN alpha 1, and mouse limitin
  • Suitable for Western Blot

C12R Mouse IFN-alpha Decoy Receptor Neutralizing activity

Representative graph obtained with the Neutralizing C12R Mouse IFN Alpha Decoy Receptor (cat. nr. 12185-1). Source: PBL / tebu-bio.

Representative graph obtained with the Neutralizing C12R Mouse IFN Alpha Decoy Receptor (cat. nr. 12185-1). Source: PBL / tebu-bio.

#4- Human IFN Lambda 3/IL-28 Neutralizing antibody

  • Mouse monoclonal antibody against Human IL-28B/IFNL3 (cat. nr 21730) – Clone MMHL-3
  • Neutralizes human IL-28B/IFNL3 bioactivity
  • Less than 0.1% cross-reactivity is observed with human IFNL1 and human IFNL2

Representative Neutralizing curve:

IFN Lambda 3 antibody titration against human IFN Lambda in A549/EMCV antiviral assay with the Human IFN Lambda 3/IL-28 Neutralizing antibody (cat. nr 21730). Source: PBL / tebu-bio.

IFN Lambda 3 antibody titration against human IFN Lambda in A549/EMCV antiviral assay with the Human IFN Lambda 3/IL-28 Neutralizing antibody (cat. nr 21730). Source: PBL / tebu-bio.

Any questions about these IFN Neutralizing reagents?

Leave your questions and comments below, I’ll be pleased to get back to you!

Written by Isabelle Nobiron, PhD
Isabelle is a Product Manager at tebu-bio, and also the company's ISO Quality Manager.