3 novel PD-L1 post-translational modifications identified

In a recent paper, Horita H. et al. identified a set of novel Post-Translational Modifications (PTMs) of the protein Programmed Cell Death Ligand (PD-L1).

Model: Profile of PD-L1 post-translational modifications and their roles in regulating PD-L1 protein levels.

Profile of PD-L1 post-translational modifications and their roles in regulating PD-L1 protein levels. Source: Horita H. et al. (2017) – http://dx.doi.org/10.1016/j.neo.2017.02.006 (Cytoskleton Inc).

For this, a novel series of PTM enrichment assays  enabling the highly specific profiling of 4 key PTM profiles (Tyrosine phosphorylation (pY), Acetylation (Ac), Unbiquitination (Ub) and SUMOylation 2/3), have been used on EGF-stimulated A431 cell line. They revealed that EGF induced pY, Ac, mono- and poly-Ub Of PD-L1 in these EGF treated epidermoid carcinoma cells. The authors also suggested that the balance between mono- / poly-Ub of PD-L1 might regulate PD-L1 stability, as already described with LDB-1 protein.

These novel PD-L1 PTMs and their related regulatory actions might be valuable information in the future for the design of drug strategies targeting PD-L1/PD-1 immune checkpoint inhibition.

 

PTM enrichement tools used in this citation (a.k.a. Signal-Seeker™ assays by Cytoskeleton Inc.):

  • Signal-Seeker™ Phosphotyrosine Enrichment Kit (Cat. nr 027BK160 – Cytoskeleton Inc.)
  • Signal-Seeker™ Ubiquitination Enrichment Kit (Cat. nr 027BK161 – Cytoskeleton Inc.)
  • Signal-Seeker™ SUMOylation 2/3 Enrichment Kit (Cat. nr 027BK162 – Cytoskeleton Inc.)
  • Acetyl Lysine Antibody Mouse Monoclonal (Cat. nr 027AAC01)
  • Rac/Cdc42 Activator II (Cat. nr 027CN02)

Related products:

PD-1 Cell Line to screen for activators or inhibitors of PD-1 signaling in a cellular context and characterize the biological activity of PD-1 and its interactions with ligands. PD-1 / NFAT Reporter - Jurkat Recombinant Cell Line (Cat. nr 14960535).

PD-1 Cell Line to screen for activators or inhibitors of PD-1 signaling in a cellular context and characterize the biological activity of PD-1 and its interactions with ligands. PD-1 / NFAT Reporter – Jurkat Recombinant Cell Line (Cat. nr 14960535).

Antibodies

  • Anti-alpha/beta tubulin: sheep polyclonal (Cat. nr 027ATN02 – Cytoskeleton Inc.)
  • Anti-PD-1 Neutralizing Antibody, PE-labeled (Cat. nr 14971290-1 – Cytoskeleton Inc.)
  • Anti-PD-1 Neutralizing Antibody (Cat. nr 14971120 – BPS Bioscience)
  • Anti-PD-1 Neutralizing Antibody, Biotin-labeled (Cat. nr 14971215 – BPS Bioscience)
  • Anti-PD-1 Neutralizing Antibody, PE-labeled (Cat. nr 14971290-2 – BPS Bioscience)

Cell-based Assays

  • PD-1:PD-L1 Homogeneous Assay Kit (Cat. nr 14972014 – BPS Bioscience)
  • PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit (Cat. nr 14972003 – BPS Bioscience)
  • PD-1[Biotinylated]:PD-L1 Homogeneous Assay Kit (Cat. nr 14972028 – BPS Bioscience)
  • PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit (Cat. nr 14972005 – BPS Bioscience)
  • PD-1[Biotinylated]:PD-L1 Inhibitor Screening Colorimetric Assay Kit (Cat. nr 14972018 – BPS Bioscience)

Source:
Horita H et al. “Identifying regulatory Posttranslational moditifcations of PD-L1: A focus on monoubiquitination (2017) Vol. 19, pp. 346-353.

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Written by Philippe Fixe, PhD
Philippe Fixe is a former Marketing Manager at tebu-bio, passionate about innovation and R&D in Life sciences, Biotechnology, Medical research, Drug discovery, and also a keen photographer.