Lympholyte®: The ideal tool for simple and viable lymphocytes isolation

High quality isolation of lymphocytes is a key step in many in vitro research programs covering immunology, infectious diseases and oncology. To simplify this step, CEDARLANE® have developed an efficient and easy-to -use cell separation media: Lympholyte®



Lymphocytes (B, T and NK cells) belong to the family of White Blood Cells (WBC or leukocytes) together with monocytes and granulocytes.  Found in the blood, bone marrow (where they are produced) and lymphoid tissues, Lymphocytes play a central role in the immune system and their blood rate might be affected during infections, genetic diseases, cancers…

Based on density gradiant centrifugation, Lympholyte® media allows isolation of viable Lymphocytes from mouse, rat, rabbit, human and other mammalian cell populations. Based on an easy cell separation procedure (Fig.1), Lympholyte® can be used for many applications:

  • Isolation of viable lymphocytes from lymphoid organs by removal of red and dead cells
  • Removal of dead cells and debris :
    • From lymphocyte suspensions after treatment with antibody plus complement or following cell culture
    • Following sequential cytotoxicity studies, eg. B-cell depletion
    • From various clonal and hybridoma cell lines

  • Removal of erythrocyte, dead cells and debris from various clonal and hybridoma cell line (Lympholyte®-M and-R)
  • Isolation of mononuclear epidermal cells (Lympholyte®-M)
  • Isolation of mononuclear cells from bone marrow preparations (Lympholyte®-H)

Fig.1: Lympholyte Cell separation procedure

The resulting cell population demonstrates high, non-selective recovery of viable lymphocytes that are suitable for use as target cells in cytotoxicity, FACS assays and in vivo / in vitro functional studies. All Lympholyte products are supplied as a sterile liquid with varying densities.

Browse the overview of all the different Lympholyte® media available:

Lympholyte®-M (Mouse)

Lympholyte®-M is a density separation medium specifically designed for the isolation of viable lymphocytes from murine lymphoid cell suspensions. It can be used to eliminate erythrocytes, dead cells and debris from murine spleen, lymph node, thymus and bone marrow suspensions.

Lympholyte®-Rat

Lympholyte-Rat is ideal for the isolation of viable lymphocytes from rat lymphoid cell
suspensions. Its simple protocol enables the elimination of erythrocytes,
dead cells and debris from rat spleen, lymph node, thymus and bone marrow
suspensions.

Lympholyte®-H (Human)

Fig. 2: Purification of peripheral blood Granulocytes with Lympholyte -H (Rondelli T, et al, PLoS One. 2013;8(1))

Lympholyte-H is designed for the isolation of viable lymphocytes and monocytes from
human peripheral and cord blood. Used for the elimination of erythrocytes and
dead cells from human blood, it can also removes the majority of granulocytes
(including neutrophils). The resulting cell population consists of a high and
non-selective recovery of viable human lymphocytes and monocytes.

Lympholyte®-Rabbit

Rabbit lymphoid cells suspensions can be cleaned with Lympholyte-Rabbit, thus eliminating erythrocytes, dead cells and debris from rabbit
spleen, lymph node and thymus suspensions.

Lympholyte®-Mammal

Lympholyte-Mammal is a density separation medium specifically designed for the isolation of viable lymphocytes and monocytes from the peripheral blood of most mammalian species. It consists of Sodium Diatrizoate combined with Dextran to induce erythrocyte aggregation and reduce platelet aggregation resulting in a higher yield of lymphocytes and monocytes. Lympholyte-Mammal can be used for the elimination of erythrocytes and dead cells from the blood of most mammalian species. Lympholyte-Mammal also removes the majority of granulocytes (including neutrophils).

Lympholyte®-Poly (Human)

Lympholyte-poly is a ready-made sterile and endotoxin tested solution which is a
mixture of sodium metrizoate and Dextran 500 that allows for the isolation of
human polymorphonuclear granulocytes from whole blood. Lympholyte-poly is
ideally suited for isolation of polymorphonuclear granulocytes (neutrophils,
eosinophils) from human whole blood. Mononuclear and polymorphonuclear
leukocytes are separated into two distinct bands free from erythrocytes. It has
also been found to be suitable for the isolation of neutrophils from bronchial
lung lavage suspension.

Lympholyte®-1.1

Lympholyte-1.1 is specifically designed for the isolation of islet cells from the
pancreas. Lympholyte-1.1 can be used undiluted to eliminate non-islet tissue
such as exocrine fragments, lymph nodes, ganglia, ductal and vascular tissue
from pancreatic cell suspension. The resulting cell population consists mainly
of islet cells that may be expanded for use in vivo.

What is your preferred solution to simplify lymphocytes isolation?

Contact your tebu-bio local office and find the Lympholyte solution the most suitable to your experiments.

Interested in Lympholytes? Browse our full offer of complementary products:

Frédéric Samazan
Written by Frédéric Samazan
Frédéric Samazan is a Product Manager at tebu-bio, and an avid football fan in his spare time.