How to obtain reliable DNA and RNA quantitation
The iQuant kits developped by ABP Bioprobes provide accurate quantitation of DNA and RNA with a wide range including high sensitivity. They are useful for NGS applications, qPCR analysis, and in vitro transcription to produce RNA.
Fluorescence-based DNA and RNA quantitation using the iQuant kits is highly sensitive. It is also specific for DNA or RNA providing more accurate concentration in the presence of common contaminants, including free nucleotides, protein, detergents and salts.
Dedicated iQuant assay kits
iQuant HS dsDNA quantitation kits provides high sensitivity measurement of the the double stranded DNA concentration. The range is 10 pg/µL – 100 ng/µL. It is very useful for subcloning, PCR product and viral DNA.
iQuant NGS-HS is the version for next generation sequencing with the same range. The iQuant™ NGS version is a specially formulated reagent. In this reagent, the dye and buffer are pre-mixed and thus ready-to-use. It is more fitted to high throughput testing.
iQuant BR dsDNA quantitation kit has a braod range 100 pg/µL – 1,000 ng/µL. It is for miniprep of DNA and genomic quantitation.
iQuant ssDNA with a range 20 pg/µL – 200 ng/µL is dedicated to quantitation of oligo, primers and denatured DNA
iQuant kits were also developped for RNA quantitation that is useful for samples of microarrays, RT-PCR and sequencing. There are 2 kits:
Excellent selectivity and sensitivity
iQuant DNA assay kits quantify DNA with excellent selectivity, and sensitivity even in the presence of RNA.
The figure above shows the DNA concentration measured with iQuant DNA kit on 3 samples: DNA only in blue, DNA+RNA in green and RNA only in red.
The fluorescent-based quantitation of the DNA with iQuant is more accurate than with Nanodrop.
iQuant is compatible with fluorescence plate reader, or a fluorometer such as Qubit®.
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