As with this painted representation of (surrealist!) DNA, to a certain extent, molecular cloning is an art.
Unfortunately for newcomers, or fortunately for experts, it’s required for many downstream applications including protein production and cell engineering to develop cell-based assays. ORF expression is also a major tool for understanding a protein function via overexpression study.
In short, to develop the potential of cell biology research, it’s often a question of adopting a “smart molecular biology” approach. Along the way though, it’s unlikely that you’ll be able to avoid using cloning vectors and other related molecular cloning tools.
However… luckily, fast and simple cloning is possible! Mainly thanks to the smart kits and systems that I’ll explain more about in this post. And for your complete peace of mind, there are also ORF expression clones…