Cell signalling and STING studies – First cyclic GAMP immunoassay now available

The innate immune system is the body’s crucial first defense against viral infections and it is dependent upon a group of pattern recognition receptors. cGMP-AMP Synthase (cGAS) responds to the presence of DNA in the cytosol by producing 2’,3’cGAMP. Cyclic GAMP in turn binds to STING leading to TBK1-mediated IRF3 activation and the production of type 1 IFN.

Understanding the STING cell signalling pathway and the role cGAMP plays in a number of important areas, such as infection, cancer, inflammation & senescence, relies on cGAMP measurement. Good news – Arbor Assays have just released a new cGAMP EIA kit, read on to learn more!

2’,3’-Cyclic guanosine monophosphate–adenosine monophosphate (cyclic GMP-AMP, cGAMP, cyclic [G(2’,5’)pA(3’,5’)p]) was the first cyclic di-nucleotide found in metazoa. 2’,3’-cGAMP is also referred to as “non-canonical“ cGAMP due to the presence of the atypical 2’-5’ phosphodiester linkage between the guanosine and the adenosine. 2’,3’-Cyclic GAMP is a novel second messenger in innate immunity that regulates type I interferon (IFN) production.

cGAMP cell signallingProduced in mammalian cells by cGAS (cGAMP synthase) in response to double-stranded DNA in the cytoplasm binding to cGAS, cGAMP binds to the stimulator of interferon genes (STING). Subsequently STING induces the TBK1-IRF3-dependent production of IFN-β. This cGAS-cGAMP-STING pathway has been shown to play a critical role in pathogen detection and physiological conditions such as metabolic dysregulation, autoimmunity, and cancer.

To provide a tool for the measurement of cGAMP, Arbor Assays have developed an immunoassay (K067), which is in fact the first one ever to be released to date.

Main features of the 2′,3′-cGAMP Direct EIA kit

  • Sensitivity – 0.08 pmol/mL, 4 fmol/well
  • Sample type – Cell Lysates, Tissue Extracts, TCM
  • Samples/Kit – 39 or 231 in Duplicate
  • Range – 50 pmol/mL – 0.205 pmol/mL
  • Time to answer –  2.5 hours

Typical data obtained

cGAMP EIA kit

A simple data analysis tool calculates concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Arbor Assays’ DetectX® 2’,3’-Cyclic GAMP EIA kit (K067-H1/H5).

Related reagents you’ll also find of interest

  • IFN beta related tools by PBL, the world’s specialist of interferon subtypes, monoclonal and polyclonal antibodies and assay kits for life science researchers
  • Cyclic AMP Direct Enzyme Immunoassay Kits (K019-H1/H5)
  • Cyclic AMP Direct Chemiluminescent Immunoassay Kits (K019-C1/C5)
  • Cyclic GMP Direct Enzyme Immunoassay Kits (K065-H1/H5)
  • Cortisol Enzyme Immunoassay Kits (K003-H1/H5)

Feel free to contact me if you’d like some advice in choosing the right cell signalling kit for your research.

Isabelle Nobiron
Written by Isabelle Nobiron
Isabelle is a Product Manager at tebu-bio, and also the company's ISO Quality Manager.