Live Cell Imaging – Watch SiR-Tubulin stained Human Osteosarcoma cells in action

As you may already know, Spirochrome have developed a broad range of Live Cell Imaging probes for the staining of DNA, Lysosome, F-Actin and Microtubules.

Based on the use of the Silicon rhodamine-like (SiR) technology, these probes meet all the requirements for easy use for all your microscopic analysis:

  • High selectivity
  • Applicable in different cell types and tissues (see my previous post for the full list)
  • Minimal toxicity
  • Excited by far-red light
  • Cell permeable
  • Suitable for Super-Resolution microscopy

More than that, SiR-Actin and SiR-Tubulin are the only stains available on the market which enable live cell imaging of the major cytoskeletal cellular structures without the need to transfect cells.

SiR-Tubulin and Human Osteosarcoma cells

As mentioned previously, SIR probes can be used with different kinds of cells and tissues.

Here, thanks to the work and the courtesy of Dr Marin Barisic, group leader at the Danish Cancer Society Research Center, we are able to show you visual data of Human Osteosarcoma cells stained with SiR-Tubulin, during the cellular division.

What you’ll see in this video…

  • 1 – Visualization of Histone H2B expressing Green Fluorescent Protein (H2B-GFP)
  • 2 – Visualization of the Tubulin stained with SiR-Tubulin (20nM + 5µM Verapamil)
  • 3 – Merge: Histone H2B (Red) and Tubulin (Cyan)

 

What about you? Interested in trying one of the SiR-Probes on your experimental model ?

Get in contact your local tebu-bio office to obtain more information or to order your SiR-probes.

 

Don’t hesitate to visit our interactive web page to easily visualize our broad offer of Live Cell Imaging tools, and find the one corresponding to your needs.

 

Frédéric Samazan
Written by Frédéric Samazan
Frédéric Samazan is a Product Manager at tebu-bio, and an avid football fan in his spare time.