Cell-based assays have become a classic way to monitor cells’ reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is Firefly Luciferase, combined or not with Renilla Luciferase. Luciferases allow simple data acquisition on a plate reader.

Nevertheless, Firefly Luciferase requires cell lysis. End-point acquisition is the only method and optimizations of the right moment is recommended. To bring more convenience, robustness and higher sensitivity, Gaussian luciferase can well be your better choice.

Hyaluronic acid (also called HA or Hyaluronan) is a glycosaminoglycan with elevated viscosity, enabling tissues (eye, skin, joint and synovial fluid…) to resist to physical and mechanical constraints (torsion, flexion…). Over time, when HA production declines, tissues progressively lose these tensile properties, leading to wrinkles and fold, weak re-epithelisation and age-related troubles. But HA is also involved in many other chronic and cancer-related diseases. In this post, we’ll review one of the most popular HA quantification assays (ELISA test), known to be highly sensitive and robust, appreciated by researchers involved in cosmetology and drug discovery.