New – 4 Unique IFN Neutralizing reagents

PBL Assay Science, the specialists of Interferon related quality reagents, have just released 4 new unique products to be used in neutralization assays. I am pleased to introduce them in this post, in which you’ll discover examples of titration data, as well as reactivity and cross-reactivity information.

#1- Human Type I IFN Neutralizing antibody mixture

  • Mixture of monoclonal and polyclonal antibodies directed against human Type 1 Interferons (IFNs) and Type 1 Receptor Subunit 2 (IFNAR2) (cat. nr 39000-1)
  • Effectively neutralizes biological activity of human IFN-alpha, IFN-beta, IFN-omega, IFN-kappa, and IFN-epsilon
  • Improves neutralization of multiple Type I IFNs often found in complex samples by employing a broadly neutralizing custom antibody cocktail
  • No cross reactivity observed with the following human

Representative neutralizing curves are shown below:

Human Type I IFN Neutralizing antibody mixture (cat. nr. 39000-1) - PBL / tebu-bio

Representative neutralize curve obtained with the Human Type I IFN Neutralizing antibody mixture (cat. nr. 39000-1). Source: PBL / tebu-bio

In this assay, A549 cells were incubated with a fixed amount of neutralizing antibody mixture and varying IFN concentrations, followed by challenging with encephalomyocarditis virus (EMCV).

#2- Human IFN Lambda 2/IL-29 Neutralizing antibody

  • Mouse monoclonal antibody against Human IL-28A/IFNL2 (cat. nr 21720) – Clone MMHL-2
  • Neutralizes human IL-28A/IFNL2 bioactivity
  • ~3% and 8% cross-reactivity is observed with human IFNL1 and human IFNL3, respectively

Representative Neutralizing curves shown below:

Representative curve obtained with the Human IFN Lambda 2/IL-29 Neutralizing antibody (cat. nr. 21720). Source: PBL / tebu-bio.

Representative curve obtained with the Human IFN Lambda 2/IL-29 Neutralizing antibody (cat. nr. 21720). Source: PBL / tebu-bio.

IFN Lambda 2 antibody tiration against human IFN Lambda in A549/EMCV antiviral assay.

#3- Neutralizing C12R Mouse IFN Alpha Decoy Receptor

  • Viral C12R IFN Antagonist selectively neutralizes all mouse IFN alpha subtypes (cat. nr 12185-1)
  • No cross-reactivity with mouse IFN beta
  • Also neutralizes all human IFN alpha subtypes, human IFN beta, human IFN omega, rat IFN alpha 1, and mouse limitin
  • Suitable for Western Blot

C12R Mouse IFN-alpha Decoy Receptor Neutralizing activity

Representative graph obtained with the Neutralizing C12R Mouse IFN Alpha Decoy Receptor (cat. nr. 12185-1). Source: PBL / tebu-bio.

Representative graph obtained with the Neutralizing C12R Mouse IFN Alpha Decoy Receptor (cat. nr. 12185-1). Source: PBL / tebu-bio.

#4- Human IFN Lambda 3/IL-28 Neutralizing antibody

  • Mouse monoclonal antibody against Human IL-28B/IFNL3 (cat. nr 21730) – Clone MMHL-3
  • Neutralizes human IL-28B/IFNL3 bioactivity
  • Less than 0.1% cross-reactivity is observed with human IFNL1 and human IFNL2

Representative Neutralizing curve:

IFN Lambda 3 antibody titration against human IFN Lambda in A549/EMCV antiviral assay with the Human IFN Lambda 3/IL-28 Neutralizing antibody (cat. nr 21730). Source: PBL / tebu-bio.

IFN Lambda 3 antibody titration against human IFN Lambda in A549/EMCV antiviral assay with the Human IFN Lambda 3/IL-28 Neutralizing antibody (cat. nr 21730). Source: PBL / tebu-bio.

Any questions about these IFN Neutralizing reagents?

Leave your questions and comments below, I’ll be pleased to get back to you!

Isabelle Nobiron, PhD
Written by Isabelle Nobiron, PhD
Isabelle is a Product Manager at tebu-bio, and also the company's ISO Quality Manager.