Human IFN ELISA test: unrivaled sensitivity and global IFN alpha detection

PBL assay specializes in the development of IFN related research Tools. One of their most appreciated assay kit by the IFN research community is the ELISA test kit for all Human IFN Alpha subtypes (41115).

Its unique features such as high sensitivity, matrix compatibility and broad detection of IFN alpha subtypes explain this popularity.

Specially optimized for autoimmune disease serum

This kit is particularly suited if you are working on Lupus, Rheumatoid Arthritis (RA), or other autoimmune diseases; with patients with circulating antibody complexes or anti-nuclear antibody (ANA). Indeed, human heterophilic antibodies in serum or plasma samples can bind to the antibodies used in an immunoassay to yield false positives or inaccurate measurements. The quantitation of IFN-alpha by this kit is not affected by these heterophilic antibodies that may be found in serum/plasma from donors with autoimmune diseases.

In a more general way, it’s suited to screening type I, II and/or III IFNs changes for clues to disease mechanism or progression (including viral infections); to monitor IFN induction as potential off-target drug effects in cell culture or patient samples, with special relevance to siRNA/RNAi studies.

High sensitivity

The assay range is within 1.95 and 125 pg/ml and lower limit of detection and quantification are shown below (see LLOQ and LLOD in pg in the table below):

 

Cross-reactivity data

The kit quantifies all human IFN-α subtypes. It does cross reacts with Cynomolgus/Rhesus IFN-α, and shows no cross reactivity with human IFN-β, IFN-γ or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; or bovine IFN-τ.

Main features and benefits for you:

  • Robust matrix tolerance for ease of use with difficult matrices
  • Low variability with less than 10% inter- and intra-assay CVs
  • Provides accurate measurement of low levels of human interferon alpha as low as ~ 1 pg/ml LOQ
  • Detects previously “missing” IFN alpha subtypes for an improved view of global IFN alpha production
  • High precision reproducibility with low variability between lots
  • Optimized for autoimmune disease serum, normal serum, and tissue culture media

As I said in the introduction, PBL is the interferon specialist and has developed several other assays.

Another exampe of a high sensitivity ELISA is the human IFN beta ELISA (cat# 41415) with an assay range within 1.2 – 150 pg/ml. It is the most sensitive human IFN beta ELISA on the market detecting to as low as 1.2 pg/ml and is also optimized for autoimmune disease samples. It has been validated by three independent CROs for sensitive measurement of IFN beta therapeutics in human serum samples.

This is to name a few only. Get in touch with me if you are looking for IFN quantification tools,  I be pleased to help you find the most appropriate one for your research, including multiplex quantification if required.

Also, if you are not an ELISA expert or need extra hands to speed up your project, tebu-bio’s own European based laboratories can run the ELISA test for you very easily… get in touch to learn more!

Isabelle Nobiron
Written by Isabelle Nobiron
Isabelle is a Product Manager at tebu-bio, and also the company's ISO Quality Manager.