Immunohistochemistry of Rabbit Anti-Wnt5A antibody. Tissue: human breast tumor. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Wnt5A antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Wnt5A is a secreted in the extracellular space and matrix. Staining: Wnt5A as a precipitated red signal with hematoxylin purple nuclear counterstain.
Written by Philippe Fixe, PhD
Philippe Fixe is Marketing Director at tebu-bio, passionate about innovation and R&D in Life sciences, Biotechnology, Medical research, Drug discovery, and also a keen photographer.