Pluripotent stem cells for improved reprogrammed Human Sebocytes

Phenocell Sebocytes display the typical epithelial morphology of primary sebocytes with heterogeneity in cell size due to lipid accumulation.

Following the start of our recent collaboration with Phenocell, we’re pleased to be able to provide high quality Sebocytes developed from Human induced pluripotent stem cells (iPSC). Thanks to a perfectly standardized reprogramming protocol, they display lower batch to batch variability, allowing better reproducibility and accuracy of your experimental results.

Sebocytes have demonstrated their large potential to be unique tools for many life science research fields such as:

  • sebocyte cell research,
  • hyper- and hypo-seborrhoea-linked disorder research,
  • pharmacology,
  • toxicology and drug discovery, including 3D skin reconstruction.

These cryopreserved reprogrammed pluripotent stem cells are available at low passage (P2), 2.106 cells/vial format and in 3 different phototypes (Caucasian, Asian and African). Developed from highly qualified Human iPS cells, each lot is validated, with a specific certificate of analysis, for all the following Sebocyte markers and specific functions.

iPS-derived human Sebocyte: morphology

Phenocell’s iPS-derived human Sebocytes display the typical epithelial morphology of primary sebocytes with heterogeneity in cell size due to lipid accumulation.

Phenocell Sebocytes from human induced pluripotent stem cells.

Phenocell Sebocytes from human induced pluripotent stem cells.

Specific marker expression

Immunohistochemistry

MUC1 - expressed in more than 80% of cells KRT7 - expressed in 80% of cells

Expression of the two Sebocyte markers: MUC1 – expressed in more than 80% of cells; KRT7 – expressed in 80% of cells

 

RT-qPCR

Functional markers are strongly expressed after 5 days:

  • Lipid activated transcription factor PPARγ, FADS2 (enzyme of the fatty acid biosynthesis pathway)
  • Androgen receptors (AR)
  • Melanocortin 5 receptor
  • K7 and MUC1
PhenoCell sebocytes with RT-qPCR - Functional markers are strongly expressed after 5 days

Evolution of specific markers in Sebocytes derived from Human induced pluripotent stem cells after 3 (d3) and 5 (d5) days in culture with the specific PhenoCULT©-SEB culture medium, compared to primary keratinocytes (Ker).

Purity – Flow Cytometry analysis

KRT7 expression shows Sebocyte purity above 90%.

PhenoCell sebocytes from Human induced pluripotent stem cells

Black: isotype control; Red: anti-KRT7 antibody

Function

Response to Linoleic acid

PhenoCell sebocytes from Human Induced Pluripotent stem cells - Response to linoleic acid

Dose-dependent (up to 5-fold) lipid accumulation (Bodipy staining), response after a 24hr treatment with linoleic acid (LA)

Response to testosterone

PhenoCell sebocytes from Human Induced Pluripotent stem cells - response to testosterone

PCi-SEB respond to a 96hr treatment with testosterone (10 µM) by a 2-fold increase in lipid content. This response is significantly inhibited by the 5α-reductase inhibitor Finasteride (10 µM).

iPS-derived human Sebocyte: viability and other parameters

  • Sebocytes reprogrammed from Human induced pluripotent stem cells: viability > 60% after thawing (Post-thaw regrowth test performed on each batch)
  • Normal Karyotype
  • All batches are tested negatively to mycoplasma before freezing
  • Additional information regarding these Human iPSC-derived Sebocytes on tebu-bio’s dedicated web page.

Interested in these Sebocytes developed from Human induced pluripotent stem cells ?

Get in touch by leaving a comment below, I’ll be pleased to get back to you to discuss your projects and needs.

Frédéric Samazan
Written by Frédéric Samazan
Frédéric Samazan is a Product Manager at tebu-bio, and an avid football fan in his spare time.