New in Live Cell Imaging: CopperGREEN and GlycoGREEN probes
One of our aims is to offer you, the researcher, a large and varied selection of biologically relevant, innovative Live Cell Imaging tools. In 2016, we started a collaboration with Goryo Chemical, when our specialists selected their unique fluorescent dyes for cellular analysis, assays and live cell imaging.
In one of my previous posts, I already invited you to take a (non-exhaustive) look at their diversified range of fluorescent dyes, which can be used in a variety of fields such as stem cell differentiation, cancer cell studies, Reactive Oxygen Species (ROS) analysis… Today, I’d like to take you through two new fluorescent probes we’re pleased to make available for you.
Specifically detect copper(I) ion
When present in excess, its redox properties turn it into a biological hazard because of its ability to generate ROS, which interferes with cellular metabolism, inducing severe disorders such as Menkes syndrome, Wilson’s disease, amyotrophic lateral sclerosis, and Alzheimer’s disease.
The CopperGREEN™ probe’s technical characteristics make it a powerful tool for specific monitoring and fluorescent imaging of Cu(I) ion in living cells.
High Specificity and reactivity to Copper(I) Ion:
Fluorescence intensity and reactivity is proportional to the Cu(I) ion concentration, in a molar ratio of 1:1.
Compatible with Fluorescence Microscopy
Detect β-galactosidase by green fluorescence
Because of its enzymatic activity, β-galactosidase is used in different life science areas such as genetics and molecular biology. Commonly used as a reporter marker for the analysis of gene expression, it can also be used as a good marker to monitor transfection efficiency.
More interestingly, it can be used as a cell aging marker by specific detection of the Senescence Associated beta-gal (SA-beta-gal) activity, specifically expressed in senescent cells.
GlycoGREEN™-βGal is a specific fluorescent probe to detect enzymatic activity of β-galactosidase and SA-β-gal and its green fluorescence can be used in combination with blue and red/orange fluorescent probes for multicolour imaging.
GlycoGREEN™-βGal has almost no fluorescence but shows strong fluorescence of >100-fold after reaction with the enzyme β-galactosidase. The reaction is rapid under typical conditions of LacZ expressing cells, 15 minutes incubation of the cells with the reagent is sufficient to detect signals with high sensitivity.
Suitable for live cell imaging
High contrast images can be obtained during live cell imaging. GlycoGREEN™-βGal has quite low nonspecific fluorescence and after the reaction with β-galactosidase, it shows strong fluorescence. Besides, it has quite low toxicity even in >10× concentration for general use during imaging of cells.
Applicable to fixed cell staining and flow cytometry
Cell permeable GlycoGREEN™-βGal can detect β-galactosidase within cells. After reaction with β-galactosidase, the generated fluorophore is retained within cells. Fluorescence is undiminished after fixation with 3% paraformaldehyde for 15 minutes.
Browse this interactive selection tool (click on the image to access it). You might also be interested in taking a look at:
Leave your comments or questions below, I’ll be pleased to get back to you!
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