New TIGIT:CD155 cell-based assay to restore immune response

immune inhibition and activation

TIGIT:CD155/CD112 immune inhibition (in green)

We previously addressed the PD-1:PD-L1/PD-L2 immune inhibition and the corresponding cell-based tools to screen immuno-oncology agents for restoring antitumor immune response. Unfortunately cancer cells have other ways to escape and survive. Let’s look together at another immune inhibition pathway, namely the TIGIT:CD155/CD112.


TIGIT:CD155/CD112 binding stops the natural killers

Human T cells and Natural Killer (NK) cells express an immunoreceptor on their surface called TIGIT, for T-cell immunoreceptor with Ig and ITIM domains. TIGIT binds proteins on the surface of dendritic cells, the CD155 and CD112. This cellular interaction stops the T cell and NK cell activation. Thus, inhibition of this binding increases immune response. Research on this inhibition is a way to develop an immune-based strategy against cancers.

Luciferase-based reporter cell lines to explore antogonists of the TIGIT:CD155 immune inhibition

Research and development to restore immune response

Antibodies such as the neutralizing antibody anti-TIGIT (by BPS) and other agents can specifically block this cell surface protein binding and activate the immune response as illustrated above and shown in the cell-based assays results below.

Anti-TIGIT Neutralizing antibody in cell-based assays

In order to test and optimize new agents, a robust TIGIT:CD155 homogeous assay will provide answer within a day.

Result from TIGIT:CD155 binding assay

Furthermore, BPS is now introducing an innovation allowing a cell-based assay closer to the in vivo situation. The teams at  BPS have established and validated (thanks notably to the neutralizing anti-TIGIT) 2 complementary reporter cell lines:

Combining them together, we get a new assay in cellular context. From now on, researchers can investigate antagonistic monoclonal antibodies targeting either TIGIT or its ligand CD155.

Positive controls, the 2 anti-TIGIT antibodies

The anti-TIGIT (71218), called inhibitor antibody can be used to block the TIGIT:CD115/CD112 binding in vitro. It is the recommended positive control for biochemical assays.

For cell-based assays, the positve control is the anti-TIGIT (71340), called neutralizing antibody. It can be use with the luciferase reporter TIGIT /NFAT cell line (60538) and the CD155/TCR Activator Cell line (60548).

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Dimitri Szymczak, PhD
Written by Dimitri Szymczak, PhD
Dimitri Szymczak is a Technical Support Specialist and Product Manager at tebu-bio, and a fan of capoeira in his spare time.