LipiDye fluorescent probe for lipid droplet monitoring in live cells

differentiated-3t3-l1-adipocytes-staines-with-oil-red-source-tebu-bio

LipiDye lipid droplet Green* is a new highly sensitive fluorescent probe for detecting lipid droplets in live or fixed cells. In this, let’s review the characteristics of this dye which is compatible with live cell imaging.

Lipid droplets store neutral lipids, and are mostly seen in the adipose tissues. The size of lipid droplets in adipocytes is >10 μm. They can be detected by conventional lipid droplet staining methods like Nile Red or Oil Red O… However, the size of lipid droplets in non-adipocytes is often too small (~0.1 μm) to be detected by these dyes.

This is where, LipiDye can be of particular interest when willing to analyze fine small lipid droplets in non-adipocyte cells or during adipogenesis.

intracellular-lipid-droplets-to-produce-a-bright-red-fluorescence-in-live-cells-with-nile-red-cat-nr-233m1441

Lipid droplets give a bright red fluorescence in live cells with Nile Red (cat nr 233M1441).

subcutaneous-adipocytes-at-two-weeks-post-differentiation-stained-with-the-neutral-lipid-dye-oil-red-o

Subcutaneous preadipocytes at two weeks post differentiation (Zen-bio). Neutral lipids stained in red with Oil Red O (cat. nr 088ST-R100.)

LipiDye lipid droplet Green in action

LipiDye lipid droplet Green (cat. nr 194FDV-0010) is selectively incorporated into lipid droplet and gives out green fluorescence (no fluorescence in solution and very limited in the cytoplasm). Non cytotoxic, LipoDye is compatible with both live for Live Cell Imaging and fixed cells (ideally with paraformaldehyde).

This fluoresencent lipid dye also display a high Signal-to-Noise ratio and photo-stability (Exc. – Em. spectrum: 405 nm – 521-530 nm).

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Live Cell Imaging Interactive web page tebu-bio

The Live Cell Imaging Interactive tebu-bio web page is available here.

*LipiDye stain is commercialized under a license from Nagoya University. (Yamaguchi E. et al. (2015) Angew Chem Int; 54;  pp: 4539-4543 – DOI: 10.1002/anie.201500229).

Keen to try LipiDye lipid droplet Green probe?

Contact me through or your local tebu-bio office or leave a message below.

Looking for more for fluorescent detection tools with live cell imaging?

You might like the following links: Interactive live cell imaging tool box to help you select your probes according to the organelle(s) or biomarker(s) of interest

 

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Philippe Fixe, PhD
Written by Philippe Fixe, PhD
Philippe Fixe is Marketing Director at tebu-bio, passionate about innovation and R&D in Life sciences, Biotechnology, Medical research, Drug discovery, and also a keen photographer.