Live cell imaging: the art of looking at biological processes in real time

Visualizing fixed cells and tissues only gives snapshots of cellular processes. To have a better insight into dynamic events ocurring in the cells or to vizualize interactions between various cellular components in real time (e.g., proteins, organelles, second messengers…), powerful microscopic approaches have been developed over the past decade. This post will review the recent live cell imaging probes developed by Goryo Chemicalsand available in Europe through tebu-bio.

In 2014, the Nobel price in chemistry was attributed to Eric Betzig, Stefan W. Hell, and William E. Moerner “for the development of super-resolved fluorescence microscopy” opening a new age for Live Cell Imaging. Besides elucidating intimate interactions of various cellular components (e.g. Actin, Tubulin and DNA), molecular probes used in live cell imaging allow to monitor accurately biological changes of various parameters in living cells (such as ion concentration variations or any other changes in the cytosol).  tebu-bio’s specialists are constantly searching for innovative and biologically relevant probes for live cell imaging applications.
Goryo Chemical tebu-bio

tebu-bio is now collaborating with Goryo Chemical to distribute in Europe their unique fluorescent dye for cellular analysis, assay, and live cell imaging.

Below a selection of the new fluorescent probes:

AcidiFluor Orange

Fig 1 Multicolor imaging using HeLa cells expressing Mitochondria-GFP: Lysosomes were stained with AcidiFluor™ ORANGE (ref. GC301) and nucleuses were stained blue with Hoechst33342.

AcidiFluor™ Series
CalFluor™ Series
GlycoFluor™ Series
Fe2x detection

Fig 2: Iron (II) detection of by FeRhoNox™-1 (ref. GC901) in HepG2 cells: HepG2 cells were treated with Fe(NH4)2(SO4)2 (FAS; 100 µM) for 0.5 hr, then FeRhoNox-1 (5 µM) was added and incubated for 1 hr.

MetalloFluor™ Series
NOFluor™ Series
ROSFluor™ Series
Cancer cell detection

Fig 3: Live cell imaging of cancer cells and HUVEC cells: Cells were treated with ProteoGREEN™-gGlu (2 µM) for 1 h (ref. GC801). The reaction was completely inhibited by treatment with GGT selective inhibitor.

ProteoFluor™ Series
StemFluor™ Series
Hypoxia detection

Fig 4: Fluorescent imaging of A549 cell under the various concentration of oxygen using MAR – Hypoxia detection probe (ref. A101-01).

HypoFluor™ Series
SuperFluor™ Series

 

 

LCI cell cartoon

Live Cell Imaging tools available at tebu-bio

If you are interested in testing one of the live cell imaging stains presented, please leave your request in the form below.

You might also like to get a complete overview about the unique tebu-bio’s Live cell imaging offer via an interactive Live cell imaging tool selection guide! On this guide, all you need is just to click on the biological parameter you want to visualize – and you’ll find the corresponding molecular probe available.

 

 

 

 

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Written by Ali El Baya, PhD
Ali el Bayâ is the Sales Manager at tebu-bio for the North of Europe.