Still using MTT or WST-1 for cell counting?
If the answer is yes, I am sure you’ll be interested to learn more about WST-8 and our Cell Counting Kit-8 (CCK-8). If the answer is no, and you’ve already switched to WST-8, just have a look at our price list… you might be surprised!
CCK-8 allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. Dojindo’s highly water-soluble tetrazolium salt, WST-8, is reduced by dehydrogenase activities in cells to give a yellow-color formazan dye, which is soluble in the tissue culture media. The amount of the formazan dye, generated by the activities of dehydrogenases in cells, is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1.
The 4 main benefits (to keep it short!)
- No toxicity to cells
- More sensitive than MTT, MTS, or WST-1
- 3 simple steps (no thawing necessary): Add – Incubate – Measure
- More stable than MTT, MTS or WST-1
Just keep on reading for more details about each of these benefits…
Cell Counting in 3 steps
Unlike MTT assays, since there is no need to lyse the cells, you don’t have to worry about the data variation.
CCK-8 is a ready-to-use solution. It is also stable at 4ºC for 1 year. Your assay can be done anytime without thaw and freeze.
CCK-8 is the highest sensitive dye for cell based assays.
Only in CCK-8 is continuous culture possible without killing cells. Below are illustrations observing Cytotoxicity after incubation with each reagent. HeLa cells were incubated with WST-8 (CCK-8 ), WST-1, or MTS and observed at 24 hours after addition of these reagents.
Well, since you’ve read this post right through to the end, I’m pleased to share this short cartoon with you!