• Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
  • Facebook
  • LinkedIn
  • Twitter
  • Vimeo
Home
Research areas
    ADME-Tox
    Biomarkers
    Cell Biology and Signalling
    Cell Sourcing - Cell Culture Technologies
    Drug Discovery
    Gene Expression - Molecular Biology
    Stem Cells
    Supplying Discovery Tools
Contact us
Meet the authors
Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Supplying Discovery Tools

Safe-Harbor, the new way to work?

21/03/2016 by Dimitri Szymczak, PhD No Comments

Stable expression in a cell lines is very useful for numerous projects. For example, it helps in Eukaryotes for optimization of protein productions and it is convenient for recurrent needs and high yields. Stable exogenous expression of tagged protein allows extensive tracking and localisation of the protein in live. Cell lines stably expressing a protein can be used for various screenings such as lost of function assays (CAS9-expressing cells), and reporter-based assays (Gaussian luciferase). Of course, the stable expression could also be shRNA for knock-down.

Stable expression implies insertion into the genome. It used to be random and could cause hits with side effects. Now, targeted insertion in Safe-Harbor sites is now possible. How can you take advantage of this progress?

Continue reading

Most popular posts

  • HeLa cells: Origin of this important cell line in life science research
  • How to choose the perfect buffer to get a pure, stabilised, functional protein
  • Monoclonal antibodies - all you need to know about antibody generation
My Tweets

Privacy & Cookies: This site uses cookies. By continuing to use this website, you agree to their use.
To find out more, including how to control cookies, see here: Cookie Policy

Copyright © 2018 - tebu-bio - visit our main website at tebu-bio.com