stable altrnative to ATRA - ec23 moleculeATRA is a well known for its ability to regulate cell differentiation. However, retinoids are excellent chromophores and efficiently absorb light and isomerise. This is not necessarily a feature required of cell culture reagents, where consistency and reliability of a product is important for robust reproducible results.

To address this issue, Reinnervate have recently developed ec23® as a pan-RAR receptor agonist that is stable, but maintains the same biological activity as ATRA.

Robust induction of Neurogenesis by ec23®

Stable alternative to ATRA - NMR ec23Reinnervate has demonstrated that synthetic retinoid ec23® is a potent inducer of pluripotent stem cell differentiation. Human TERA2.cl.SP12 pluripotent stem cells exposed to either 10 μM ec23® or ATRA form populations of terminally differentiated neurons. Immunocytochemical staining for neurofilament-200 was performed on differentiated cultures and the number of positive cells quantified. Results show that ec23® is a more potent inducer of neurogenesis than ATRA. In direct comparison to ATRA, they found that synthetic retinoid ec23® induces larger numbers of neural cells with less variability and with the added advantage of compound stability.

Synthetic retinoid ec23® is also a potent inducer of neurogenesis by adult neuroprogenitor cells. Rat adult hippocampal progenitor cells (AHPCs) exposed to either 1 μM ec23® or ATRA differentiate into neural cell types. Differentiation induced by exposure to ec23® was consistent and reproducible.

Product Specifications

Size 10 mg (Cat. No. SR-P002-2)
Alternate Name  4-(5,5,8,8-Tetramethyl-5,6,7,8-tetrahydronaphthalen-2-ylethynyl) benzoic acid
Chemical Formula  C23H24O2
Molecular Weight  332
CAS Number  104561-41-3
Purity Greater than 99% by HPLC analysis
Formulation Off-white solid
Solubility Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.
Storage and Stability Store powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed.
Quality Control The purity of ec23 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry.

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Written by Jean-François Têtu, PhD
Jean-François Têtu is Sales Manager at tebu-bio for the south of Europe and also for the UK and Eire. He also fervently defends and promotes anything from his home territory, the north of France.