• Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
  • Facebook
  • LinkedIn
  • Twitter
  • Vimeo
Home
Research areas
    ADME-Tox
    Biomarkers
    Cell Biology and Signalling
    Cell Sourcing - Cell Culture Technologies
    Drug Discovery
    Gene Expression - Molecular Biology
    Stem Cells
    Supplying Discovery Tools
Contact us
Meet the authors
Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Supplying Discovery Tools

9 reagents to visualize actin in fixed cells

31/12/2015 by Philippe Fixe, PhD No Comments
Cystoskeleton #1 - F-actin visualized with Acti-stain™ 488 (PHDG1) in Swiss 3T3 cells treated with CN03 Rho Activator (DNA staining with DAPI (blue)). Cytoskeleton Inc.

2015 was marked by the increasing number of studies related to cellular cytoskeletal dynamics. In this context, it should be noted that the new generation of live cell-imaging SiR-Actin & SiR-Tubulin stains have been widely embraced by Life Scientists. Launched a few months ago, these fluorescent probes are now regularly cited in the “Material & Methods” section of numerous prestigious publications as indicated by Ali (see his the posts “Live cell imaging tool SiR-actin on JBC frontcover” and “User experience of SiR-Actin and SiR-Tubulin Live Cell Imaging“).

Nevertheless, cytoskeletal interactions are still widely studied in “fixed” cells. In the same manner as for live cell-imaging, these experimental assays require high quality reagents too. With the help of our friends from Cystoskeleton Inc., we have selected 9 premium research tools validated for actin visualization in PFA-fixed cells.

Continue reading

Most popular posts

  • HeLa cells: Origin of this important cell line in life science research
  • How to choose the perfect buffer to get a pure, stabilised, functional protein
  • How to make the challenge of mRNA delivery easy
My Tweets

Privacy & Cookies: This site uses cookies. By continuing to use this website, you agree to their use.
To find out more, including how to control cookies, see here: Cookie Policy

Copyright © 2018 - tebu-bio - visit our main website at tebu-bio.com