58 different human RTKs in one phosphorylation array

Phosphorylation tebu-bio

The Receptor Tyrosine Kinases (RTKs) are an important family of cell-surface receptors transmitting extracellular stimuli (ex. NGF, PDGF, FGF, EGF, Insulin…) into intracellular protein-tyrosine kinase activity, which subsequently induces a signal-transduction cascade. This signal transduction pathway leads to the regulation of cellular proliferation, differentiation, and metabolism, but also to gene expression modulation and Ras activation (a GTPase switch protein).

A link has been shown between an excessive activation of RTK signaling pathways and numerous diseases and cancers. The development of new generation drugs which target RTK activity is a new challenge with the need to use convenient but reliable methods. Phospho-specific antibodies in ELISAs or array-based systems are ideal tools for protein phosphorylation studies. RayBiotech‘s Phosphorylation arrays enable researchers to discover receptors and phosphorylation pathways activiated in their experimental models.

Human RTK Phosphorylation Array C1

The RayBio®  Human RTK Phosphorylation is specifically designed to identify simultaneous relative levels of phosphorylation of 58 different human RTKs and 13 closely related proteins regulated by tyrosin phosphorylation.

tebu-bio - Human Phospho-RTK Array

Typical results obtained with the Human Phospho-RTK Array – tebu-bio

 

The Human Phospho-RTK Array detects tyrosine-phosphorylated receptors in A431 cell lysates. Representative images of The Human Phospho-RTK Array applied with cell lysates of A431 cells untreated (A) or treated with 100 ng/mL recombinant human EGF for 10 minutes (B). 200 µg of lysate was run on each array. Quantitative results of pixel density of phosphorylation of EGFR, ErbB2, ErbB3, and PDGFRb are also shown (C). RTK Phosphorylation Array target map is shown in (D). POS=positive control (biotinylated IgG); NEG=negative control (buffer); Blank (no spot).

Source: Raybiotech

 

 

 

RTKs are key regulators of important cell cellular processes like proliferation, differentiation, metabolism and migration.

The RayBio® Human EGFR (Epidermal Growth Factor Receptor) Phosphorylation Antibody Array is specifically designed to focus on site-specific activation of the EGFR Family.  This Antibody Array utilizes 17 phospho-specific antibodies targeting specific residues on the EGF receptor, allowing detection of a highly detailed phosphoryaltion signature.

phospho ERGFR - tebu-bio

Typical results obtained with phospho ERGFR – tebu-bio

 

Detection of relative phospho-EGFR levels in A431 cells following stimulation with EGF. (A) Analysis of cell lysates using Human Phospho-EGFR (S1070) and pan EGFR ELISA kit (Cat# PEL-EGFR-S1070-T). (B) Immunoblot analysis of the lysates using antibodies against phospho-EGFR (S1070) and EGFR (the same antibodies used in the ELISA kit).

Source : Raybiotech

 

 

 

RayBio®  Phosphorylation ELISA kits and Antibody Arrays are very convenient, rapid and sensitive 126 Certified service provider with logosmethods for monitoring phosphorylation. Theses products are available as ready-to-use kits or lab services. To ensure top quality in the data obtained, Raybiotech’s service providers successfully complete training and certification programs to receive the “RayBiotech Certified Array Service Providers” award. In Europe, tebu-bio laboratories (France) were among the first laboratories in the world to be certified by Raybiotech in 2012.

 

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Written by Elodie Monin
Community Manager