Fluorescence thermometer of living HEK293 cells

Thousands of researchers spend time pampering their primary cells and cell lines every day, with the aim of defining their optimal cell culture conditions. Researchers are in effect trying to control numerous cellular parameters (oxygene levels, fresh media supply, incubation temperature…) and monitor their “viability” on a regular basis. But what about the internal cellular temperature? Have you ever considered this biological parameter to monitor the “wellness” of your cultivated cells? In this post, let’s take a look at the characteristics of a new, cell permeable, fluorescent thermometer for living cells.

The Cellular Thermoprobe for Fluorescence Ratio is a fluorescent polymeric thermometer that gives you data about the intracellular temperature distribution in living cells. Delivered inside the cells by diffusion (without microinjection), this thermoprobe is compatible with both adherent and suspension cells. It enables you to distinguish intracellular regional temperature of organelles in your cultured mammalian cells just by monitoring a fluorescence ratio.

See Cellular Thermoprobe in action

Fluorescence Thermometer in living HEK293 cells. Cellular Thermoprobe for Fluorescence (from left to right): Confocal fluorescence image, fluorescence image at 490-530 nm, 570-610 nm and merged data. Bar: 20μm. tebu-bio

Fluorescence Thermometer in living HEK293 cells. Cellular Thermoprobe for Fluorescence (from left to right): Confocal fluorescence image, fluorescence image at 490-530 nm, 570-610 nm and merged data. Bar: 20μm.

Example of calibration Curve in MOLT-4 cells - Fluorescent Cellular Thermoprobe. tebu-bio

Fluorescent thermometer ratio and temperature resolution (open, right axis) in MOLT-4 cells. The temperature resolution of Cellular Thermoprobe for Fluorescence Ratio was 0.01 – 0.25℃ in the temperature range between 25 and 44℃.

Looking for experimental procedures to  generate a calibration curve, monitor intracellular temperature in adherent or suspension cells?

The Cellular Thermoprobe is easy-to-use! You just need to dilute it in the media and and the stain is taken into cells by diffusion.

The readout does not require Fluorescence Lifetime for data acquisition, and it brings sensitive temperature resolution (0.01-0.25 °C).

 

Fluorescent Spectrum of Cellular Thermoprobe for Fluorescence Ratio inside living cells – 37°C (red), 32°C (yellow), 27°C (blue). tebu-bio

Fluorescent Spectrum of Cellular Thermoprobe for Fluorescence Ratio inside living cells – 37°C (red), 32°C (yellow), 27°C (blue).

Intracellular temperature in HEK293T Cells detected with Cellular Thermoprobe for Fluorescence Ratio. tebu-bio

Intracellular temperature in HEK293T Cells detected with Cellular Thermoprobe for Fluorescence Ratio.

Want to know more the Cellular Thermoprobe for Fluorescence Ratio?

Download the Cellular Thermoprobe for Fluorescence Ratio datasheet here.

You can also leave a message below or subscribe to our Cell Sourcing – Cell culture Technologies e-Newsletter to stay up to date on new tools such as this one!

Philippe Fixe, PhD
Written by Philippe Fixe, PhD
Philippe Fixe is Marketing Director at tebu-bio, passionate about innovation and R&D in Life sciences, Biotechnology, Medical research, Drug discovery, and also a keen photographer.