Fig.1: Collagen source and Sircol assay choice.
Matrix components are key R&D elements in pharmaceutical and cosmetology with tremendous impacts on tissue regeneration, skin repair, wound healing, allergy and inflammation, surgery, ageing diseases…
Collagen, Elastin, and Proteoglycans are major components of the Extracellular Matrix (ECM) where they form highly complex networks. ECM builds up an environment which supports surrounding cells and plays a crucial role in cell-to-cell communication, cell adhesion, and differentiation.
The monitoring of ECM components during experimental approaches requires robust and user-friendly in vitro assays. In the past experimental methods (e.g. soluble collagen measurement) were laborious and based on the use of harmful chemicals. Today, easy-to-use and straightforward methods with colorimetric read-outs are used daily.
