CRISPRCas9 genome editing complex

CRISPR/Cas9 is relatively simple to implement, as the researcher fully controls the experimental design of the tools, from the sgRNA sequence to the Cas9 protein.

Let’s quickly look back to the basics… CRISPR Associated Protein 9 (Cas9) functions as part of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) genome editing system.
In this system, a single guide RNA (sgRNA) sequence targets a site of interest in the genome while the Cas9 protein performs a double stranded DNA cleavage at the targeted genomic location. CRISPR/Cas9 system can be widely used to express or silence a gene, create mutations and variants… into the genome of the host cell.

We’re regularly contacted by researchers regarding our offer of fully processed mature mRNAs designed for genome edition. Among all the questions received, the one frequently asked is: Which Cas9 mRNA should I choose for optimal CRISPR gene editing experiments?”

Well, The 5-methylcytidine & pseudouridine modified version of our Cas9 mRNA is one of our best sellers.

What makes our Cas9 mRNA (5mC, Psi) CRISPR Associated Protein 9 mRNA so popular?

This Cas9 mRNA (5mC, Psi) encodes the Streptococcus pyogenes SF370 Cas9 protein with codon optimization for expression in mammalian systems.
CRISPR/Cas 9 Genome editing and Engineering

CRISPR/Cas 9 Genome editing system.

Capped (Cap 0) and polyadenylated, the transcript contains pseudouridine (psi) to decrease nuclease activity and Toll-Like Receptor activation and 5-MethylCytidine (5mC) to reduce the stimulation of the innate immune response. C- and N-terminal Nuclear Localization Signals (NLS) are present to increase the frequency of delivery to the nucleus and thus the rate of DNA cleavage. Additionally, a C-terminal HA-tag is added to support better detection and purification of the Cas9 protein.
Are you also keen to use fully mature Cas9 mRNAs, or any other pre-designed mRNAs for CRISPR genome editing?
Contact your local tebu-bio experts or leave a message below!

 

Philippe Fixe, PhD
Written by Philippe Fixe, PhD
Philippe Fixe is Marketing Director at tebu-bio, passionate about innovation and R&D in Life sciences, Biotechnology, Medical research, Drug discovery, and also a keen photographer.