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Gene Expression - Molecular Biology, Headlines, Supplying Discovery Tools

Two new enzymes available to replace TAP

03/09/2015 by Mark Livingstone 1 Comment

We have been closely following the interesting case of the discontinuation of tobacco acid pyrophosphatase (TAP) and the efforts of the world’s RNA biologists to find a suitable alternative. Our previous post gives a bit of the background and discusses how protocols for 5′ RACE, transcriptional start site (TSS) mapping and GRO-Seq require a good decapping enzyme to remove cap structures from RNAs to allow oligocapping. Here we discuss two new commercial offerings to replace the discontinued TAP enzyme and show some user data comparing the 3 enzymes.

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