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    ADME-Tox
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Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
News

Hello from Krisztina in Milan, a Research Travel Grant winner!

30/06/2015 by Elodie Monin No Comments

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Here are some photos sent by one of our April Travel Grants Winners !

She is just back from the 39th Annual Meeting of the European Working Group on Cardiac Cellular Electrophysiology in conjunction with the EHRA Europace-Cardiostim Congress in Milan.

 

 

 

 

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Gene Expression - Molecular Biology

CRISPR genome editing: which cell line to choose?

by Mark Livingstone No Comments

Many labs have adopted the CRISPR genome editing technology to make knock-out and knock-in cell lines.

This technology produces first a targeted break in genomic DNA, which can then be exploited to produce cell lines with genes knocked out or where a donor vector has been used to introduce new genetic elements (point mutants, fluorescent tags, antibiotic resistance cassettes, etc.). Essentially any desired modification to the cells genome can be made. In setting up these genome editing projects there are many choices to be made including vector for the Cas9 protein and for the sgRNAs. Perhaps the most difficult choice, however, can be which cell line to use. Even the most affordable stable genome editing cell line development services can come with a significant cost, so choosing the right cell line at the beginning is crucial. Here we explain some of the choices researchers have in setting up their CRISPR genome editing projects and give our advice for cell line selection.

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