Recently, the non-FRET EnSens technology was launched for in vitro assessment of specific protease activities (Enzium, Inc.). Up to date, the EnSens substates were validated for the detection of protease activity in microplate assays. Now, Enzium has extended the applicability of their Ensens method to 3 D live-cell imaging. On the top of this, they announce that the experimental procedure is easy and non-toxic for cell cultures and co-cultures. So how does live-cell imaging help you locate protease activities in in vitro tumor invasion assays ?
Epicentre® (an Illumina Company) has developed a series of MasterPure™ DNA and RNA purification kits for nearly any sample type. While Epicentre’s QuickExtract™ DNA Extraction Solution is designed for the fastest extraction of low abundance DNA for PCR, the MasterPure™ kits yield the highest quality DNA and RNA for applications such as:
- NGS genomic DNA and RNA-Seq library preps
- DNA methylation/epigenomics studies
- Genomic DNA and cDNA cloning
- qPCR and qRT-PCR
These kits offer a simple protocol for the purification of Genomic DNA, cellular RNA, or total nucleic acid (TNA):
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