The U.S.-based company KromaTiD, Inc. offers a a large collection of catalog and custom chromosomal imaging paints, probes, and kits based on their patented technology. KromaTiD appears to have the only method capable of detecting so-called cryptic inversions where relatively small segments of the genome have changed orientation. Other technologies have a lower limit of inversion detection of 10 megabases, however KromaTiD’s directional Genomic Hybridization (dGH) technology allows visualization of inversions as small as 6 kilobases.
KromaTiD’s dGH kits can be directly integrated into any lab performing traditional chromosome analyses and fluorescence in situ hybridization (FISH). The assay is performed on living cells, which undergo one replication cycle in the presence of a DNA analog. The photolabile analog daughter DNA strand can then be destroyed by light leaving only the parent strand, thus allowing directional chromatid-specific imaging with KromaTiD’s probes.