To dilute or not to dilute biological samples? And if yes, how much? That’s the question!

Following our post on whether samples should be pooled or not when performing studies for Biomarker discovery, it’s now time to discuss sample dilution (yes or no) and dilution factor (how much) when performing proteomic analysis of Biomarkers.

Many researchers are facing a dilemma: they want to set up a CRISPR genome editing project but they can’t decide which cell line to use for genome editing. Even some of the most cost-effective genome editing cell line generation services like the one from GeneCopoeia will cost a few thousand euros, so picking the correct cell line and setting up the project correctly is very important. Researchers working in primary cells may find the idea of switching to an immortalized cell line a bit artificial. They dream of the possibility of editing the genome of a stem cell line which they can then differentiate into their tissue of choice as needed.