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tebu-bio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
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    • ADME-Tox
    • Biomarkers
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    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
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Gene Expression - Molecular Biology

What's new in qPCR?

11/02/2015 by Mark Livingstone No Comments

We know molecular biologists spent a lot of time setting up their qPCR protocols and aren’t about to change the way they do things. We also know they are technically savvy and like keeping up with the recent advances, so we prepared this brief update about what’s new in the world of qPCR.

Molecular biologists know that in qPCR, you generally have a choice between the dye-based (e.g. SYBR® Green) protocols and the hydrolysis (e.g. TaqMan® MGB) probes. Most researchers have a general impression that it takes a good amount of expertise to set up good qPCR assays with a dye-based approach and that, in the hands of an expert, a SYBR® Green approach is the only way to have truly quantitative PCR. The hydrolysis probe-based approach is thought to be more end-user friendly and will yield reasonably trustworthy results even if you don’t have the world’s expert on qPCR working in your lab and is also the best for multiplexing. The thing that both qPCR methods have in common is that users are generally fairly loyal to their technique, so people tend to have ignored the innovations that have occurred in the field of qPCR over the past several years. Here, we’ll try to bring you a brief history of the past few years of qPCR innovation.

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Biomarkers

Biomarker profiling or multiplex quantification for everyone

by Nathalie Bervas No Comments

Many researchers would be keen to identify new targets for their research project: add a new cytokine to the classical inflammatory panels, find the missing link between 2 phosphorylation pathways, dig into the miRNA to find a new therapeutic target…
They expect they’ll need dedicated (and expensive) new equipment. Not necessarily! Let’s take a look at assays that use existing and quite common readers, or that can easily be outsourced to reliable labs…

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